Article
Extended Data Fig. 8 | Fluorescence microscopy of tobacco alkaloid
transporters expressed in CSY1296 for alleviation of vacuolar TA transport
limitations. a, b, C-terminal GFP fusions of NtJ AT 1 (a) and NtMATE2 (b) were
expressed from low-copy plasmids in CSY1296. Strains were cultured, imaged,
and analysed via widefield epifluorescence microscopy and 2D deconvolution
analysis as described in the Methods. Images are representative of two
independent experiments. Scale bar, 5 μm.