Extended Data Fig. 8 | Substrate scopes for BACED and pySOOF. a, A
comprehensive list of all side chains installed into proteins with the BACED
reaction manifold. General conditions: protein 1 mg ml−1; 50 W, 450 nm light;
4 °C to RT; 100–1, 500 equiv. BACED precursor reagent; 10 equiv. Cat1 or Cat2;
100 equiv. catechol; <6 ppm O 2 ; pH 6.0 buffer (500 mM NH 4 OAc or PBS ± 3 M
GdnHCl). b, A comprehensive list of all side chains installed into proteins with
the pySOOF reaction manifold. General conditions: protein 1 mg ml−1; 50 W,
450 nm light; RT; 2–5 equiv. pySOOF precursor reagent; 0.4–4 equiv. Cat1;
50–100 equiv. FeSO 4 ; <6 ppm O 2 ; pH 6.0 buffer (500 mM NH 4 OAc or various
other buffers).