Nature - USA (2020-09-24)

3

nature research | reporting summary

October 2018

(WB). Citations on CiteAb Database for the FG4R clone (from different supplier, ThermoFisher): 42

Monoclonal Anti-polyHistidine-Alkaline Phosphatase: Source and Use: Sigma-Aldrich, catalogue number A5588, clone HIS-1, lot

number 085M4836V, dilution 1:2,000. [ Supplier link: https://www.sigmaaldrich.com/catalog/product/sigma/a5588?

lang=en®ion=GB] Supplier verification: Antibody tested by western blot, no other validation given. Supplier suggested

applications: Western Blot (WB). Citations on CiteAb Database: 11

Histone H3 Antibody: Source and Use: Cell Signaling Technology, catalogue number 3638S, clone 96C10, lot number 10, 1:1,000

[ Supplier link: https://www.cellsignal.co.uk/products/primary-antibodies/histone-h3-96c10-mouse-mab/3638 ] Supplier

verification: Antibody tested by western blot, no other validation given. Supplier suggested applications: Western Blot (WB),

Immunoprecipitation (IP), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Immunofluorescence (IF), Flow

Cytometry (F), ELISE-Peptide (E-P). Citations on CiteAb Database: 118

Goat Anti-Mouse IgG H&L Alkaline Phosphatase: Source and Use: Sigma-Aldrich, catalog number A3562, polyclonal, lot number

SLCB8722, dilution 1:10,000. [ Supplier link: https://www.sigmaaldrich.com/catalog/product/sigma/a3562?lang=en®ion=GB ]

Supplier verification: No verification given. Supplier suggested applications: ELISA (ELISA), Dot Blot (DB), Immunohistochemistry

(IHC), Western Blot (WB). Citations on CiteAb Database: 176.

Anti-Mouse IgG (H+L) HRP Conjugate: Source and Use: Promega, catalogue number W4021, polyclonal, lot number 0000306114,

dilution 1:2,500 [ Supplier link: https://www.promega.co.uk/products/protein-detection/primary-and-secondary-antibodies/

anti_mouse-igg-h-and-l-hrp-conjugate/?catNum=W4021 ] Supplier verification: Tested by western blot and ELISA, extensively

used and published in numerous research articles, no other verification given. Supplier suggested applications: Western Blot

(WB), ELISA (ELISA), Dot Blot (DB). Citations on CiteAb Database: 248

Eukaryotic cell lines

Policy information about cell lines

Cell line source(s) Adherent HeLa WT cell line were sourced from the European Collection of Authenticated Cell Cultures (ECACC, supplied by

Sigma-Aldrich, catalogue number 93021013, lot number 17A011).

Authentication The Adherent HeLa WT cell line underwent 1 passage and cells were used unauthenticated before they were used to

generate HeLa cell nuclear lysate.

Mycoplasma contamination The cell line tested negative for mycoplasma contamination

Commonly misidentified lines

(See ICLAC register)

No commonly misidentified cell lines used