Plant Biotechnology and Genetics: Principles, Techniques and Applications

(Brent) #1
But, research is also about people. The
value of those who have served as my
mentors along the way cannot be under-
stated. I must mention Glenn Collins at
the University of Kentucky, in whose
lab I did an undergraduate research
thesis, to whose lab I returned for a
postdoc. Glenn has never stopped
being my chief mentor. Then there is
Richard Smith, my major professor
from graduate school. I still see his
work ethic and research approach in
everything I do. Finally, I want to

single out Roger Boerma and Joe
Bouton at the University of Georgia—
two established faculty members who
helped me out every step of the way.

Last but not least, I have had the good
fortune to have had many postdocs,
graduate students, and undergraduate
students whose thought-provoking ques-
tions and enthusiasm have led my
research forward. Seeing them move on
and progress in their own careers has
been as rewarding as the research itself.

References


Ait-ali T, Rands C, Harberd N (2003): Flexible control of plant architecture and yield via switchable
expression of Arabidopsis gai.Plant Biotechnol J 1 :337–343.
Brand L, Ho ̈rler M, Nu ̈esch E, Vassalli S, Barrell P, Yang W, Jefferson RA, Grossniklaus U, Curtis
MD (2006): A versatile and reliable two-component system for tissue-specific gene induction in
Arabidopsis.Plant Physiol 141 :1194–1204.
Brodersen P, Voinnet O (2006): The diversity of RNA silencing pathways in plants.Trends Genet
22 :268–280.
Butaye KMJ, Goderis IJWM, Wouters PFJ, Pues JM-TG, Delaure ́SL, Broekaert WF, Depickers A,
Cammue BPA, De Bolle MFC (2004): Stable high-level transgene expression in Arabidopsis
thaliana using gene silencing mutants and matrix attachment regions.The Plant J 39 :440–449.
Castle LA, Siehl DL, Gorton R, Patten PA, Chen YH, Bertain S, Cho HJ, Duck N, Wong J, Liu D,
Lassner MW (2004): Discovery and directed evolution of a glyphosate tolerance gene.Science
304 :1151–1154.
Clark AJ, Warren GJ (1979): Conjugal transmission of plasmids.Annu Rev Genet 13 :99–125.
Curtis MD, Grossniklaus U (2003): A gateway cloning vector set for high-throughput functional
analysis of genes in planta.Plant Physiol 133 :462–469.
Curtis MD, Grossniklaus U (2006): Conditional gene expression in plants. In Floriculture,
Ornamental and Plant Biotechnology: Advances and Topical Issues, Vol II, Teixera da Silva
JA (ed). Global Science Books London, pp 77–87.
Eguchi Y, Itoh T, Tomizawa J (1991): Antisense RNA.Annu Rev Biochem 60 :631–652.
Fu X, Sudhakar D, Peng J, Richards DE, Christou P, Harberd NP (2001): Expression of Arabidopsis
GAI in transgenic rice represses multiple gibberellin responses.Plant Cell 13 :1791–1802.
Goderis IJ, De Bolle MF, Francois IE, Wouters PF, Broekaert WF, Cammue BP (2002): A set of
modular plant transformation vectors allowing flexible insertion of up to six expression units.
Plant Mol Biol 50 :17–27.
Hartley JL, Temple GF, Brasch MA (2000): DNA cloning using in vitro site-specific recombination.
Genome Res 10 :1788–1795.
Joshi L, Lopez LC (2005): Bioprospecting in plants for engineered proteins.Curr Opin Plant Biol
8 :223–226.
Joubes J, De Schutter K, Verkest A, Inze D, De Veylder L (2004): Conditional, recombinase-mediated
expression of genes in plant cell cultures.Plant J 37 :889–896.


190 RECOMBINANT DNA, VECTOR DESIGN, AND CONSTRUCTION
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