TOFG-all

(Marcin) #1
Soil Biology & Ecology

Unit 2.3 | Part 2 – 115

Demonstration 4: Soil Arthropods


for the instructor


OVERVIEW


This demonstration introduces


students to techniques for sampling


soil arthropods and familiarizes


them with their functional roles.


For this short demonstration, both


of these exercises provide a hands-


on, show-and-tell of soil arthropods.


You should have identification keys


available, and some familiarity with


what kinds of animals students are


likely to find.


A pitfall trap is buried so the top sits


flush with the soil surface, allowing


surface-dwelling arthropods to fall


in. Preserving liquid can be used in


the bottom of the trap to keep the


arthropods there for easy removal


and identification. A Tullgren


funnel uses light to dry out a soil


or compost sample, driving out


organisms so they can be collected


and identified.


MATERIALS

PITFALL TRAPPING
Ê UÊ
Õ«ÃÊ­i°}°]ÊxääʜÀÊ£äääʓÊ«>Ã̈VÊ`Àˆ˜ŽÊVÕ«ÃÆʣʫiÀÊÌÀ>«ÊˆvÊ
one-time installation, or 2 per trap if you wish to repeat the
ÌÀ>««ˆ˜}®
Ê UÊ /ÀœÜi
Ê UÊ ­"«Ìˆœ˜>®Ê*ÀiÃiÀۈ˜}Ê>}i˜Ì]Êi°}°]Êi̅>˜œÊœÀÊ«Àœ«Þi˜iÊ
glycol, especially for demonstrations that will run several
`>ÞÃʜÀʓœÀiÊvÀœ“ÊÃÌ>ÀÌÊ̜Êw˜ˆÃ…°Ê9œÕʘii`Êi˜œÕ}…ÊvœÀÊ
x‡£äÊV“ʈ˜Ê̅iÊLœÌ̜“ÊœvÊi>V…ÊÌÀ>«°Ê

TULLGREN FUNNELS
Ê UÊ ՘˜iÃÊ­£ÊvœÀÊi>V…Ê/Տ}Ài˜Êv՘˜iÊÃi̇ի®†
Ê UÊ ˆ}…ÌÊÜÕÀViÊ­{Ê̜Ê{äÊÜ>ÌÌÊpÇÊÜ>ÌÌʺ
…ÀˆÃ̓>ûÊÃÌޏiÊ
ˆ}…ÌÃÊܜÀŽÊÜiÆÊi>V…Êv՘˜iÊ˜ii`ÃʈÌÃʜܘʏˆ}…ÌÊÜÕÀVi®
Ê UÊ Õ“ˆ˜Õ“ÊvœˆÊ­ˆvʏˆ}…ÌÊÜÕÀViÊ`œiÃʘœÌʅ>ÛiÊ>ÊÅ>`iÊvœÀÊ
vœVÕȘ}Ê̅iʅi>Ìʜ˜Ê̅iÊÃ>“«i®
Ê UÊ -VÀii˜Ê­£Ê«ˆiViÊ«iÀÊv՘˜i®‡
Ê UÊ >ÀÃÊ­£Ê«iÀÊv՘˜i®
Ê UÊ
̅>˜œÊœÀÊ«Àœ«Þi˜iÊ}ÞVœ°Ê9œÕʘii`Êi˜œÕ}…ÊvœÀÊ£äqÓäÊ
cm in the jar below the funnel.
†Ê -Ìii«‡Ãˆ`i`Êv՘˜iÃÊ܈̅ʘœÊÃi>“ÃÊܜÀŽÊÜiÆʈ˜ÛiÀÌi`ÊÜ`>Ê
bottles work, and inverted polypropylene Erlenmeyer flasks
܈̅ÊLœÌ̜“ÃÊÀi“œÛi`Ê>ÀiÊiÝVii˜Ì°Ê1ÃiÊxääʓÊy>ÎÃÊvœÀÊ
5 xÊxÊV“Ê܈ÊVœÀiÃ]Ê>˜`ÊÓäääʓÊy>ÎÃÊvœÀÊVœ“«œÃÌʜÀÊ
litter samples.
‡Ê
Ý>“«iÃÊvœÀÊÃVÀii˜Ê“>ÌiÀˆ>Ê>ÀiÊw˜iʅ>À`Ü>ÀiÊVœÌ…ÊœÀÊ
«>Ã̈Vʘii`iܜÀŽÊL>VŽˆ˜}pˆÌÊŜՏ`ÊLiÊw˜iÊi˜œÕ}…Ê̜Ê
>œÜÊÓ>ÊœÀ}>˜ˆÃ“ÃÊ̜ʫ>ÃÃÊ̅ÀœÕ}…Ê­i°}°]ʏiÃÃÊ̅>˜Ê£‡V“Ê
œ«i˜ˆ˜}î°Ê/…iÊÃVÀii˜ÊŜՏ`ÊLiÊÌÀˆ““i`Ê̜ÊwÌÊ>VÀœÃÃÊ̅iÊ
mouth of or in the middle the funnel, as its role is to hold
the sample in place in the funnel while it dries out.

FOR BOTH METHODS
Ê UÊ ˆÃÃiV̈œ˜Ê“ˆVÀœÃVœ«iÃʜÀʅ>˜`ʏi˜ÃiÃ
Ê UÊ `i˜ÌˆwV>̈œ˜Ê}Ո`i]Êi°}°]Ê
http://www.cals.ncsu.edu/course/ent525/soil/soilpix/index.html
Ê UÊ -œˆÊÀ̅Àœ«œ`Ê >Ì>Ê-…iiÌÊ­««i˜`ˆÝÊÈ®

Instructor’s Demonstration 4 Outline

Free download pdf