Medical Microbiology

Cultivation 623

Serum

&Antibodiestovariousparasites: 2 – 5 mlserumor 5 – 10 mlofwholeblood

(bothwithoutadditives)(seealsoTable 11. 5 ,p.625).

Cerebrospinalfluid

&AntibodiestoTaeniasolium(suspectedcysticercosis): 1 – 2 mlofcere-

brospinalfluid,noadditives.

&Trypanosomes: 1 – 2 mlofcerebrospinalfluid,noadditives.

BronchialSpecimens

&MicrosporaandPneumocystiscarinii:inducedsputumor 20 mlof

bronchiallavage.

Urine

&Schistosomaeggs andmicrosporidia:sediment(about 20 ml)from

24-hoururine.

Cultivation

&Visceralleishmaniosis:sampleobtainedunderasepticconditionsby

puncturefromlymphnodesorbonemarrowmustbetransferredimmedi-

atelytoculturemedium(orderfromlaboratory).

&Cutaneousleishmaniosis:takespecimentissuefromtheedgesofthe

lesion(followingsurfacedisinfection)andtransfertoculturemedium.

&Acanthamoebas: 1 – 2 mlofcontactlensrinsingliquidorconjunctival

lavage,noadditives.

11

Kayser, Medical Microbiology © 2005 Thieme