ImmunodiagnosticandMolecularTechniques 625
Table 11. 5 ImmunologicalandMolecularDiagnosisofParasitosesinHumans:
ASelectionofTechniquesandEstablishedMethods
Parasitosis Methods
Antibodyassay^1 Antigenassay DNAanalysis
Africantrypanosomosis
(sleepingsickness)
IFAT,ELISA,HA PCR(blood)
Americantrypanosomosis
(Chagasdisease)
IFAT,ELISA,HA PCR(blood)
Leishmaniosis
&visceral IFAT,ELISA PCR(blood,lymph
nodeaspirate)
&cutaneous/mucocuta-
neous
(IFAT,ELISA) PCR(biopsy)
Giardiosis IFAT,ELISA(stool)
Amebosis(Entamebosis)
&intestinal ELISA,IFAT ELISA(stool) PCR(stool)
&extraintestinal ELISA,IFAT
Toxoplasmosis ELISA,IFAT,SFT,
CFT,ISAGA,WB,
IgGaviditytest
PCR(amniotic
fluid,placenta,
etc.)
Cryptosporidiosis ELISA,IFAT(stool)
Malaria IFAT Rapidtest
(blood)^2
PCR(blood)
Microsporosis PCR(stool,urine,
etc.)
Schistosomosis IFAT,ELISA
Fasciolosis IFAT,ELISA ELISA(stool)
Opisthorchiosis ELISA
Paragonimosis ELISA,HA
Echinococcosis ELISA,IFAT,WB PCR
(metacestodes)
Cysticercosis WB,ELISA
Taeniosis ELISA(stool) PCR(proglottids)
Toxocarosis ELISA,WB
Filariosis ELISA,IFAT ELISA(serum)
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Kayser, Medical Microbiology © 2005 Thieme