Medical Microbiology

Table 1 2. 9 HematopoieticandLymphoreticularSystem

Infection Mostimportant

pathogens

Laboratorydiagnosis

HIVinfection

(AIDS)

HIV-1;HIV-2 Serology:EIAandWesternblot.

Alsop2 4 antigenassayforprimary

infection.Quantitativegenometest

withRT-PCRfortherapeuticindica-

tionandcourse(viralload).

Infectious

mononucleosis

Epstein–Barrvirus(EBV)

Cytomegalovirus(rare)

Serology

Isolationfromurineandsaliva;

serology

Brucellosis Brucellaabortus

Brucellamelitensis

Brucellasuis

Bloodculture:threesetsfrom

threedifferentsites,within 1 – 2 h,

beforeantimicrobialsifpossible.

10 – 20 mlvenousbloodintoone

aerobicandoneanaerobicbottle,

respectively.

Incubationforupto 4 weeksis

necessary—informlaboratoryof

suspectedBrucellainfection.

Serology

Tularemia Francisellatularensis Culturefromlymphnodebiopsy,

sputumandblood;serology

Plague Yersiniapestis Microscopyandculturefrom

bubopus,possiblyfromsputum

(pulmonaryplague)

Melioidosis Burkholderiapseudomallei Microscopyandculturefrom

sputum,abscesspusorblood

Malleus(glanders) Burkholderiamallei Microscopyandculturefromnasal

secretion,abscesspusorblood

Rat-bitefever Streptobacillusmoniliformis Culturefromlesionspecimen

Sodoku Spirillumminus Attemptmicroscopicaldetectionin

bloodorwoundsecretion

648 12 EtiologicalandLaboratoryDiagnosticSummariesinTabularForm

12

Kayser, Medical Microbiology © 2005 Thieme