137B
BIOLOGICAL TREATMENT OF WASTEWATER
- INTRODUCTION
Biological treatment is the most widely used method for
removal, as well as partial or complete stabilization of bio-
logically degradable substances present in waste-waters.
Most often, the degradable substances are organic in nature
and may be present as suspended, colloidal or dissolved
matter. The fraction of each form depends on the nature of
wastewater. In the operation of biological treatment facili-
ties, the characteristics of wastewater are measured in terms
of its chemical oxygen demand, COD, biochemical oxygen
demand, BOD, total organic carbon, TOC, and volatile sus-
pended solids, VSS; concepts of which have been discussed
elsewhere.^1
Most of the conventional biological wastewater treat-
ment processes are based on naturally occurring biologi-
cal phenomena, but are carried out at accelerated rates.
These processes employ bacteria as the primary organ-
isms; however, certain other microorganisms may also
play an important role. Gates and Ghosh^2 have presented
the biological component system existing in the BOD pro-
cess and it is shown in Figure 1. The degradation and sta-
bilization of organic matter is accomplished by their use
as food by bacteria and other microorganisms to produce
protoplasm for new cells during the growth process. When
a small number of microorganisms are inoculated into a
bacteriological culture medium, growth of bacteria with
time follows a definite pattern as depicted in Figure 2 by
plotting viable count and mass of bacteria against time.^3
The population dynamics of bacteria in biological treat-
ment processes depends upon various environmental fac-
tors including pH, temperature, type and concentration
of substrate, hydrogen acceptor, availability and concen-
tration of essential nutrients like nitrogen, phosphorous,
sulfur, etc., and essential minerals, osmotic pressure, tox-
icity of media or by-products, and degree of mixing.^4 In
recent years, cultures have been developed for biological
treatment of many hard-to-degrade organic wastes.- METABOLIC REACTIONS
The metabolic reactions occurring within a biological treat-
ment reactor can be divided into three phases: oxidation, syn-
thesis and endogenous respiration. Oxidation–reduction may
proceed either in the presence of free oxygen, aerobically,
or in its absence, anaerobically. While the overall reactionsSUBSTRATEORGANICS
OXYGEN
GROWTH
FACTORS
LYSISED
PRODUCTSOXYGEN
BACTERIA
(PRIMARY FEEDERS)DEAD
BIOMASSAUTO-
DESTRUCTIONOXYGENGROWTH
FACTORSCO 2CO 2H 2 OH 2 OENERGYENERGYOTHER PRODUCTSOTHER
PRODUCTSCO 2
H 2 O
ENERGY
OTHER
PRODUCTSP R O T O Z O AB A C T E R I APROTOZOA
(SECONDARY FEEDERS)FIGURE 1 Biological component system existing in BOD process.C002_001_r03.indd 137C002_001_r03.indd 137 11/18/2005 10:15:47 AM11/18/2005 10:15:47 AM