Biology Questions and Answers

Biology Questions and Answers

patrimony to the organism owner of the
cell whose nucleus was used in thetransplantation.

9. What is PCR? How does PCR
   works?

PCR, polymmethod to syntherase chaiesize man reaction, ny copies ofis a (^)
specific regions oknown as target-regions. If a DNA mts inventor,olecule (^)
Kary Mullis, won the Nobel prize for
Chemistry in 1993.
First, the DNA to be tested is heated to
cause the double helix to rupture andthe polynucleotide chains to be
exposed. Then small synthetic
sequences oand containing f DNA knucleotide sequencesnown as primers (^)
similarextremities to the sequences of the of the region to be studied (^)
(for example, a region containing a
known gene exclusive oorganism) are added. The primersf a given (^)
paired with the original DNA in the
extremitiesEnzymes known as polym of the gene to be amplified.erases, that
catalyze DNA replication, and nucleotide
supply arcompleted and the chosen region ise added. The primers then are
replicated. In the presence of more
primers acopies of that spnd more nucleotides millions oecific region are f
generated. (PCR is very sensitive even
using a minimal amount of DNA).

10. What is the fact of
    Molecular Biology on which
    DNA fingerprint is based?

DNA fingerprint, the method of

individual based on the fact that the DNA identification using DNA, isof every (^)
individual (with exception of identical
twins annucleotidd individual clones) coe sequences exclusive to entainsach (^)
individual.
Although normal individuals of the samespecies have the same genes in their
chromosomes, edifferent alleles anach individual hd even in the inactiveas (^)
portions of the chromosomes
(heterochromatin) thin nucleotide sequences amonere are differencesg
individuals.

11. Why are the recombinant
    DNA technology and the
    nucleus transplantation
    technology still dangerous?

The recombinant DNA tthe nucleus transplantation techechnology andnology (^)
(cloning) are extremely dangerous sincethey are able to modify, in a very short
time, the ecological balaevolution has taken millionsnce that of years to (^)
create on the planet. During the
evolutionary and gradual action ofprocess, under the slow mutations, genetic (^)
recombinations and of natural selection
species emgenetic patrimonierged and were modified andes were formed. With
genetic engineering however humans
can mix and modify gechanges of unpredictable lones, makingng term (^)
consequences, risking creating new
plant or cancers aanimalnd new disease diseases, noutbreaks. Itew types of (^)
is a field as potentially dangerous as the
manipulation of nuclear energy.
http://www.biology-questions-and-answers.com