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Chapter 20 REVIEW

702 Chapter 20 NEL

Many of these questions are in the style of the Diploma
Exam. You will find guidance for writing Diploma Exams in
Appendix A5. Science Directing Words used in Diploma
Exams are in bold type. Exam study tips and test-taking
suggestions are on the Nelson Web site.

DO NOT WRITE IN THIS TEXTBOOK.

Part 1

1. Identify the DNA sequence that would result in
   phenylalanine being placed in a polypeptide chain.
   A. UUG
   B. AAC
   C. UUU
   D. TTT

2.Identify the term that best describes the mutation that

causes the loss of phenylalanine.

A. silent mutation

B. insertion mutation

C. deletion mutation

D. missense mutation

3.Gene therapy trials to correct this defect in the CFTRgene

have been conducted by doctors in several centres. The

following is a list of some genetic technologies that might

be used in this work:

1. restriction endonucelases


2. mtDNA


3. polymerase chain reaction


4. DNA ligase


5. viruses


6. bacterial plasmids


7. gene sequencing
   Identify the technologies that would most likely be used to
   isolate the gene for a therapy trial. (Record all four digits of
   your answer in the order in which the technologies would
   be used.)

4.Identify the enzyme that is correctly matched with its

function.

A. DNA polymerase I: synthesis of the continuous

matching strand

B. DNA helicase: synthesis of messenger RNA

C. DNA polymerase III: cuts out the primer and replaces

it with DNA nucleotides

D. DNA ligase: links adjacent nucleotides together by

covalent bond

5.Select the response that correctly identifies the

complementary DNA strand for this strand:

5 
-TACTTTGGCCCAGAG-3


A. 3 
-AUGAAACCGGGUCUC-5


B. 3 
-UACUUUGGCCCAGA-5


C. 3 
-ATGAAACCGGGTCTC-5


D. 5 
-ATGAAACCGGGTCTC-3

Use the following information to answer questions 1 to 3.

The cause of cystic fibrosis has been identified as a variety of

mutations to the CFTRgene on chromosome 7. The most

common of these involves the loss of three nucleotides, which

in turn results in the loss of a phenylalanine at amino acid

position 508. Use the following information to answer questions 6 and 7.

1. Amino acids are brought to the ribosome and linked
   together in the correct order.


2. A copy of the gene is taken to the ribosome.


3. RNA polymerase attaches to the promoter site.


4. The two subunits of the ribosome attach to the RNA strand.


5. DNA polymerase III makes a matching strand using
   complementary base pairs.


6. Release factor binds to the A site and the ribosome
   releases the amino acid chain.


7. The two original strands serve as templates for the
   synthesis of new matching stands.


8. The lagging strand is synthesized in short fragments.


9. The two strands are unwound and the hydrogen bonds are
   broken.

6.Identify the steps described above that correspond to the

process of replication. (Record all four digits of your answer

in the order the steps would occur in the cell.)

7.Match these terms to the selection above that best

describes them. (Record all four digits of your answer.)

________ ________ ________ ________

initiation of termination elongation initiation of

transcription of translation of amino translation

acid chain

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