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cell or tissue produces a major protein of the cell a large fraction of the total mRNA will code for the protein. An example of ...

a requirement for the cloning of PCR-amplified DNA. For example certain techniques such asin vitroprotein synthesis are best ach ...

6.3 Cloning vectors For the cloning of any molecule of DNA it is necessary for that DNA to be incorporated into a cloning vector ...

capacity). Vectors have in general been developed from naturally occurring molecules such as bacterial plasmids, bacteriophages ...

initiated far more frequently than chromosomal replication. Hence a large number of plasmid molecules will be produced per cell. ...

plasmid that has recircularised without an insert, dimers of plasmid, fragments joined to each other, and plasmid with an insert ...

plasmids with inserts. Since replica plating gives an identical pattern of colonies on both sets of plates, it is straightforwar ...

fragment of DNA, a non-functional enzyme results which is unable to carry out hydrolysis of X-gal. Thus, a recombinant pUC plasm ...

upon entry to the cell, phage head and tail proteins synthesised rapidly and new functional phage assembled. The phage are subse ...

asymmetric they are similar to restriction sites and allow the phage DNA to be circularised. Phage may be replicated very effici ...

lZapis a commercially produced cloning vector that includes unique cloning sites clustered into a multiple cloning site (MCS) (F ...

product rather than the DNA inserted into the vector. This screening is therefore undertaken with antibody probes directed again ...

The helper phage displaces a strand within thelZap which contains the foreign DNA insert. This is circularised and packaged as a ...

RF DNA. Replication then proceeds rapidly until some 100 RF molecules are produced within theE. colicell. DNA synthesis then swi ...

mp18/19, etc., all of which have a number of highly useful features. All contain a synthetic MCS, which is located in thelacZgen ...

especially useful for the analysis of highly complex genomes and are an important part of various genome mapping projects (Secti ...

between thecossites is 52 kb. Consequently cosmid vectors have been constructed that incorporate thecossites from phageland also ...

6.3.7 Vectors used in eukaryotes The use ofE. colifor general cloning and manipulation of DNA is well established; however, nume ...

This means that constructs may be prepared rapidly in the bacteria and delivered into yeast for expression studies. The bacteriu ...

cells, because of their ease of growth and short doubling time. Gram-negative bacteria such asE. colican be madecompetentfor the ...