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Table 1.1SI units – basic and derived units Quantity SI unit Symbol (basic SI units) Definition of SI unit Equivalent in SI unit ...

35.5 (Cl) i.e. 58.5, so that one mole is 58.5 grams. If this was dissolved in water and adjusted to a total volume of 1 dm^3 the ...

1.3 WEAK ELECTROLYTES 1.3.1 The biochemical importance of weak electrolytes Many molecules of biochemical importance are weak el ...

the amino acids, peptides, proteins, nucleosides, nucleotides and nucleic acids. It also includes the reagents used in the prepa ...

This reversible reaction can be represented by an equilibrium constant,Ka, known as theacid dissociation constant(equation 1.3). ...

hence pKaþpKb¼pKw¼ 14 ð 1 : 6 Þ This relationship holds for all acid–base pairs and enables one pKavalue to be calculated from k ...

biochemical examples is phosphoric acid, H 3 PO 4 , as it is widely used as the basis of a buffer in the pH region of 6.70 (see ...

1.3.3 Buffer solutions A buffer solution is one that resists a change in pH on the addition of either acid or base. They are of ...

Example 2BUFFER ACTION AND pH OF BLOOD The normal pH of blood is 7.4 and is maintained at this value by buffer action in particu ...

Selection of a buffer When selecting a buffer for a particular experimental study, several factors should be taken into account: ...

ensure that the proposed buffer has other desirable properties such as being non-toxic, able to penetrate membranes, and does n ...

1.3.5 Other electrodes Electrodes exist for the measurement of many other ions such as Liþ,Kþ,Naþ,Ca^2 þ, Cland NO 3 in additi ...

1.4 QUANTITATIVE BIOCHEMICAL MEASUREMENTS 1.4.1 Analytical considerations and experimental error Many biochemical investigations ...

immunological methods such as ELISA. Once a method has been selected it must be developed and/or validated using the approaches ...

characteristic, however, is that their cause can be identified and corrected. There are four common means of identifying this ty ...

of test analyte. If the replicated values agree closely with each other, the measurements are said to be ofhigh precision(orlow ...

of the ability of the method to give a consistent negative result for known negatives (see Section 16.1.2) Analytical sensitivi ...

three cases the term is actually measuring the width of the normal distribution curve such that the narrower the curve the small ...

CV¼ s 100 % x ð 1 : 12 Þ Since the mean and standard deviation have the same units, coefficient of variation is simply a percent ...

Example 4ASSESSMENT OF THE PRECISION OF AN ANALYTICAL DATA SET Question Five measurements of the fasting serum glucose concentra ...