9780521516358book.pdf

instruments are still important for solid phase sequencing to identify post-translational modifications; in particular, sites of ...

9.5.4 Carbon isotopes and finding the charge state of a peptide Since the mass detector operates on the basis of mass-to-charge ...

calculated from its sequence as 1295.69. The experimentally derived values are, for the singly charged ion, [(MþH)/1]¼1296.65 an ...

Example 6PEPTIDE MASS DETERMINATION (III) Question An unknown peptide and an enzymatic digest of it were analysed by mass spectr ...

‘Delta Mass’, which is a database of protein post-translational modifications that can be found at http://www.abrf.org/index.cfm ...

9.5.6 Selected ion monitoring Selected ion monitoring(SIM) is typically used to look for ions that are characteristic of a targe ...

HPLC–ES MS. Glycopeptides can be identified by characteristic fragment ions includ- ing hexoseþ(163 Da) andN-acetyl hexosamineþ( ...

1368.45(M +Na) + 1370.46 1249.55 1248.53 1271.61 1390.44 1244.54 1272.60 1392.45 1155.53 1398.52 1225.59 1146.60 1067.62 1441.44 ...

can also be carried out to specifically bind one particular component in a mixture. Immobilised metal ion affinity columnsare us ...

isolated from cells or tissues in different states. The two samples are combined and proteolysed, normally with trypsin, for rea ...

9.7 Computing and database analysis 9.7.1 Organic compound databases Mass spectrometry organic compound databases are available ...

NIST Chemistry WebBook. NIST is the National Institute for Standards and Technology. Data on specific compounds in the Chemistr ...

theoretical and the experimental mass of a particular peptide) which should be low and consistent. This gives an indication of w ...

form of carbon. This simplifies the spectrum but more importantly this will ensure that the search algorithm will not be not con ...

Deltamass is a database of protein post-translational modifications at http://www.abrf.org/index.cfm/dm.home which can be access ...

10 Electrophoretic techniques J. WALKER 10.1 General principles 10.2 Support media 10.3 Electrophoresis of proteins 10.4 Electro ...

electrophoresis unit. All electrophoresis is carried out in an appropriate buffer, which is essential to maintain a constant sta ...

In order to understand fully how charged species separate it is necessary to look at some simple equations relating to electroph ...

Most of this power generated is dissipated as heat. Heating of the electrophoretic medium has the following effects: An increas ...

10.2 Support media The pioneering work on protein electrophoresis by Arne Tiselius (for which he received the Nobel Prize in Che ...