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a larger uncertainty associated with them. The uncertainty of the sample mean deviating from the population mean decreases in th ...

(and hence with the varying degrees of freedom) at selected confidence levels are available in statistical tables. Some values a ...

Example 5ASSESSMENT OF THE ACCURACY OF AN ANALYTICAL DATA SET Question Calculate the confidence intervals at the 50%, 95% and 99 ...

test is based on anull hypothesis, namely that there is no difference in the values being compared. If the hypothesis is proved ...

Each test is based on a null hypothesis, which is that there is no difference between the means of the two data sets. The tests ...

Comparing two competitive analytical methods – unpairedt-test In quantitative biochemical analysis it is frequently helpful to c ...

or computer packages (Table 1.9). If the calculated value ofFis less than the table value, the null hypothesis is proved and the ...

Comparison of two competitive analytical methods – pairedt-test A variant of the previous type of comparison of two analytical m ...

differences between the results of each method for each test sample. This is an example of a pairedt-test. The formula for calcu ...

Example 9COMPARISON OF TWO ANALYTICAL METHODS USING DIFFERENT TEST SAMPLES Question Ten fasting serum samples were each analysed ...

them (so they are all positive), sums them and then selects the values ofmandcthat give the minimum deviations. The end result o ...

This may be achieved automatically in spectrophotometric measurements by the use of a double-beam spectrophotometer in which the ...

that careful thought be given to all aspects of safety before the experimental design is finalised. Health hazards come from a v ...

other emergency services. It is normal for all laboratories to have a nominated Safety Officer whose responsibility it is to giv ...

2 Cell culture techniques A. R. BAYDOUN 2.1 Introduction 2.2 The cell culture laboratory and equipment 2.3 Safety considerations ...

exploitation of which avoids the legal, moral and ethical questions generally associated with animal experimentation. More impor ...

generated should be disposed of immediately. The disposal procedure may require prior autoclaving of the waste, which can be car ...

over the tissue culture (Fig. 2.1). This mechanism protects the operator and ensures that the air over the cultures is largely s ...

water), which acts against bacteria and fungal spores by dehydrating and fixing cells, thus preventing contamination of cultures ...

preferably on the inside of the inner glass door. This can then be checked on a regular basis and adjustments made as required. ...