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a labelled DNA probe is where you think it is (and not down the sink!) or checking chromatographic fractions for labelled compon ...

Dynodes absorb fast-moving electrons and create larger number of slower-moving electrons etc. Light-sensitive surface emits phot ...

Advantages of scintillation counting Scintillation counting is widely used in biological work and it has several advantages over ...

window ofB, it is possible to separately count the two isotopes. A pulse height analyser set with a threshold and window for a p ...

As a sample is quenched, the efficiency of light production falls, and it therefore creates an illusion that the radiation has a ...

chapter but the chances of either three or two tubes detecting a signal is affected by the extent of quench. Therefore the ratio ...

to the effluent prior to entering a detector. This significantly increases automation and reduces waste. Cˇerenkov counting TheC ...

There are many applications of this technology such as enzyme assays and receptor binding, indeed any situation where we want to ...

Choice of emulsion and film Autoradiography emulsions are solutions of silver halide that can be made to set solid by the inclus ...

Intensifying screens Intensifying screens are used when obtaining a fast result is more important than high resolution. It is us ...

efficiency in a scintillation counter will not correct for self-absorption effects. Particulate samplesshould be digestedorother ...

Consider these simple examples for a series of 1 min counts: counts¼ 100 ¼ ffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffif ...

work is shown in Table 14.5. The key factors in the decision are often based on safety, the type of detection to be used, the se ...

Although they undergo the same reactions, different isotopes may do so at different rates. This is known as the isotope effect. ...

Currently the dose limit for workers exposed to radiation is 20 mSv in a year to the whole body, but this is rarely ever approac ...

and gases); most work in biology involves manipulations of radioactive liquids. Control of contamination is assisted by: comply ...

A useful guide for internal risks is the annual limit on intake (ALI). The ingestion of one ALI results in a person receiving a ...

15 Enzymes K. WILSON 15.1 Characteristics and nomenclature 15.2 Enzyme steady-state kinetics 15.3 Analytical methods for the stu ...

monitored to aid diagnosis of the organ damage and to make a prognosis for the patient’s future recovery (Section 16.3). Enzymes ...

Group 6:Ligases(also calledsynthases), which catalyse covalent bond formation with the concomitant breakdown of a nucleoside tr ...