9780521516358book.pdf

intensity is measured parallel (i.e. in the same plane) to the absorbed plane-polarised light and at right angles to it. From th ...

Protein microarray technology This approach to the study of receptor–ligand binding exploits the principle that assay systems th ...

systems are rapid and relatively easy to interpret, cellular assays have several advantages: they do not require any pre-purifi ...

the likely substrates of the very large number of protein kinases involved in cell signalling and to study the role of ubiquitin ...

capable of acting on an added substrate of the enzyme to release a fluorescent or chemiluminescent signal that can readily be de ...

structure, but with someb-structure. Eacha-helical region has been designated TM1–TM4 and the experimental evidence supports the ...

receptor structure and desensitisation. Mutagenesis studies of these serine residues have shown that their replacement by non-po ...

to be distributed in specific regions of the brain. In the resting state the channels are closed, but the binding of glutamate l ...

near the N-terminal end for the C-terminal region of the receptor and a guanine nucleotide-binding site that also possess GTPase ...

Binding of the GPCR–G-protein complex to the G-protein–adaptor complex induces an allosteric conformational change in the guani ...

GPCR dimerisation Evidence has been obtained to indicate that GPCRs interact with other GPCRs to form either homo- or heterodime ...

other ‘closed’, and that the role of glutamate is to stabilise the closed, active form. In contrast, the GABABreceptor is a hete ...

Fourthly, the dimerisation affords the potential for further allosteric modification of ligand (agonist, G-protein, other prote ...

(RGS). This group of over 20 proteins can be divided into five subfamilies based on sequence homology. They all have two actions ...

occur within less than 50 ms, that the activation of the G-protein and its interaction with the effector protein occur within 50 ...

Subclass I EGF-R Subclass II Insulin-R Subclass III PDGF-R I II I II Binding domain Cys-rich domain Binding domain Cys-rich doma ...

Binding of insulin to its binding site (one on each of theachains) causes a conformational change that activates the kinase acti ...

on each monomer forming SH2 or phosphotyrosine binding (PTB) domains that serve as docking sites for effector proteins that poss ...

immunological, inflammatory and infectious diseases. They have also been termed lymphokines, interleukins and chemokines. They a ...

the dissociation of the Gasubunit from the Gbgsubunit allowing the Gasubunit to initiate signalling, for example by activating a ...