9780521516358book.pdf

2.6.4 Culture of human embryonic stem cells Once the feeders are ready, hESCs can be plated directly by depositing the suspensio ...

2.6.5 Enzymatic subculture of hESCs As with standard cell culture, hESCs can be passaged using enzymes but in this case an enzym ...

microscope in a tissue culture hood. Undifferentiated colonies are identified by their morphology and then cut into grids (see F ...

for sustaining growth. The conditioned medium can be generated by incubating normal growth medium with feeder cells for 24 h bef ...

2.7 BACTERIAL CELL CULTURE As with animal cells, pure bacterial cultures (cultures that contain only one species of organism) ar ...

substances, including meat and yeast extract, and as a result are less well defined, since their precise composition is largely ...

inoculating an aliquot of cells into a sterile flask containing a finite amount of medium. Such systems are referred to as close ...

2.7.5 Determination of growth of bacterial cultures Several methods are available for determining the growth of bacterial cells ...

2.9 SUGGESTIONS FOR FURTHER READING Ball, A. S. (1997).Bacterial Cell Culture: Essential Data. John Wiley & Sons, Inc., New ...

3 Centrifugation K. OHLENDIECK 3.1 Introduction 3.2 Basic principles of sedimentation 3.3 Types, care and safety aspects of cent ...

applications. To overcome this problem, the description of preparative centrifugation techniques is accompanied by an explanator ...

the denser the biological buffer system is, the slower the particle will move in a centrifugal field; the greater thefrictional ...

and therefore the centrifugal field can be expressed as: G¼^4  (^2) ðrev min (^1) Þ^2 r 3600 ¼^4  (^2) s (^2) r 3600 ð 3 : 3 ...

100 000 95 000 90 000 85 000 80 000 75 000 70 000 65 000 60 000 50 000 40 000 30 000 20 000 10 000 200 1 000 000900 000 800 000 ...

whereis the sedimentation rate of the sphere, 2/9 is the shape factor constant for a sphere,ris the radius of particle,rpis the ...

macromolecules are relatively small, and are usually expressed (see Section 3.5), as Svedberg units, S. One Svedberg unit equals ...

in design and are mainly used to collect small amounts of biological material, such as blood cells. To prevent denaturation of s ...

switched on at least an hour prior to the centrifugation run. On the other hand, modern ultracentrifuges can be started even wit ...

Centrifugal field Centrifugal field Centrifugal field Axis of rotor Axis of rotor Axis of rotor rav rmin rmax rav rmin rmax rav ...

Fixed-angle rotors are an ideal tool for pelleting during the differential separation of biological particles where sedimentatio ...