Fig. 4.17Multiple labelling in a living mouse brain using the ‘Brainbow’ technique. Unique colour combinations
in individual neurons are achieved by the relative levels of three or more fluorescent proteins (XFPs). The
images are collected using a multi-channel laser scanning confocal microscope. Up to 90 different colours
(neurons) can be distinguished using this technique. Top image, hippocampus; bottom image, brainstem.
(Image courtesy of Jeff Lichtman, Harvard University, USA.) (See also colour plate.)125 4.4 Imaging living cells and tissues