9780521516358book.pdf

(a) (b)

(c)

Fig. 4.25Integrated microscopy. (a) Epifluorescence image and (b) and (c) whole mount TEM at different

magnifications of the same cell. The fluorescence image is labelled with rhodamine phalloidin, which stains

polymerised actin. A stress fibre at the periphery of the cell appears as a white line in the fluorescence image

(a), and when viewed in the TEM the stress fibres appear as aligned densities of actin filaments. The TEM whole

mount was prepared using detergent extraction, chemical fixation, critical point drying and platinum/carbon

coating. (Image kindly provided by Tatyana Svitkina, University of Pennsylvania, USA.)

Table 4.4Websites of interest

http://www.microscopyu.com/

http://www.microscopy.fsu.edu/

http://www.microscopy-analysis.com/

http://www.msa.microscopy.org

http://www.rms.org.uk/index.shtml

http://www.peachpit.com/articles/article.aspx?p=1221827

http://www.openmicroscopy.org

http://swehsc.pharmacy.arizona.edu/exppath/micro/index.html

http://www.itg.uiuc.edu/

http://rsb.info.nih.gov/ij/

http://www.openmicroscopy.org

135 4.7 Image archiving