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in particular has been used to correct the defective ion transport by introducing a

wild-type cystic fibrosis gene into cells bearing a mutated cystic fibrosis (CFTR) gene.

There is no doubt that the further development of these vector systems will enhance

eukaryotic protein expression in the future.

6.7.2 Phage display techniques

As a result of the production of phagemid vectors and as a means of overcoming the

problems of screening large numbers of clones generated from genomic libraries of

antibody genes, a method for linking the phenotype or expressed protein with the

genotype has been devised. This is termedphage display, since a functional protein is

Expression of protein fused with 6  His-Tag

Nickel-chelate-nitrilotriacetate

(Ni-NTA) chromatography column

Protein-His-Tag cleavage

Recovery and purification of protein

His His His His His His His

His

His

His

His

His His His

His His His His His His His

Fig. 6.36Recovery of proteins using (6His-Tag) and (Ni-NTA) chromatography columns.

238 Recombinant DNA and genetic analysis