4321
Fig. 10.14Photograph showing four tracks from a 0.8% agarose submarine gel. The gel was run at 40 V in Tris/
borate/EDTA buffer for 16 h, stained with ethidium bromide and viewed under ultraviolet light. Sample loadings
were about 0.5mg of DNA per track. Tracks 1 and 2,lDNA (49 kb). Track 3,lDNA cleaved with the enzyme
EcoRI to generate fragments of the following size (in order from the origin): 21.80 kb, 7.52 kb, 5.93 kb, 5.54 kb,
4.80 kb, 3.41 kb. Track 4,lDNA cleaved with the enzymeHindIII to generate fragments of the following size (in
order from the origin): 23.70 kb, 9.46 kb, 6.75 kb, 4.26 kb, 2.26 kb, 1.98 kb. (Courtesy of Stephen Boffey,
University of Hertfordshire.)424 Electrophoretic techniques