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Table 11.4Stationary phases commonly used for exclusion

chromatography

Polymer Trade name

Fractionation rangea

(Mr 10 –3)

Low-pressure liquid chromatography

Dextran Sephadex

G-10 <0.7

G-25 1–5

G-50 1.5–30

G-100 4–150

G-200 5–600

Dextran, cross-linked Sephacryl

S-100 1–100

S-200 5–250

S-300 10–1500

S-400 20–8000

Agarose Sepharose

6B 10–4000

4B 60–20 000

2B 70–40 000

Polyacrylamide Bio-Gel

P-2 0.1–1.8

P-6 1–6

P-30 2.5–40

P-100 5–100

P-300 60–400

High-performance liquid chromatography

Polyvinyl chloride Fractogel

TSK HW-40 0.1–10

TSK HW-55 1–700

TSK HW-65 50–5000

TSK HW-75 500–50 000

464 Chromatographic techniques