9780521516358book.pdf

SFM-400 Stopped-flow mode

Motor

Mixer

Motor Motor

Delay line Mixer

Exit

Cuvette

Light Detection

Delay line

Mixer

Motor

S1 S2 S3 S4

SFM-400 Quenched-flow mode

Motor

Mixer

Motor Motor

Delay line Mixer Delay line

Mixer

Motor

S1 S2 S3 S4

Collect Waste

Valve

Fig. 15.12The BioLogic stopped-flow and quenched-flow apparatus. The reactants are placed in

separate syringes each driven by a microprocessor-controlled stepping motor capable of delivering 0.01 to

10.00 cm^3 min^1 with a minimum injection volume of 10–30 mm^3. The reactants are pre-mixed before they

606 Enzymes