Index
absolute specificity 581
accreditation of clinical
laboratories 639
accuracy 19
acidic ion-exchanger 460
acrylamide 405
activation energy 597
active antiretroviral therapy 724
activity, specific 317, 574
acute phase response 658
adenine 140
adenosine 140 triphosphateseeATP
adenylyl cyclase 662, 693, 703
adherent cells 52–3
adjuvants 273
A-DNA 147
adsorption chromatography 453–5
Advisory Committee on Dangerous
Pathogens 44
affinity
chromatography 324
constant 586, 667
development 435
purification (by centrifugation)
93–5
agar 404
agarose gels 404–5, 422
agglutination 284
agonists 660, 664–7
inverse 665
partial 664
protean 669
Agrobacterium tumefaciens 222
albumin 656
alcohol dehydrogenase 583
alkaline phosphatase 646
allele-specific oligonuceotide PCR
249–50
allelic forms 146
allosteric
enzymes 599–601, 616
regulation (receptors) 665, 670–1
alpha
particles 556, 560
amino acid
analysis 330
ionisation of 301–3
single-letter code 301
three-letter code 301
ammonium sulphate precipitation 322
amphipathic 661
ampholytes 412
amplification (enzymes) 619
analysers (mass spectrometry) 359–77
analytes 433
analytical
centrifugation 95–9
methods 16, 18
range 19
specificity 19
sensitivity 20
angular velocity 76
anion exchanger 459
anisotropy 683
annealing 145
antagonists (receptor) 665
antibodies
anti-idiotype 279
chimeric 298
classification of 271–2
fragmentation of 270
humanised 298
labelling of 288–9
monoclonal 267–9, 274–6, 280–2
polyclonal 267–8, 273–4
production of 268, 273–6
purification of 281–2
primary 420
quantitation 282
recombinant 269
secondary 420
single-chain 279
structure 270–2
therapeutic 297–9
anticodon 160
antigen 264, 265
antigenic determinantseeEpitope
apparent volume of distribution 714
applied centrifugal field 75
arraysseemicroarrays
Arrhenius equation 596
ARS element 220
aseptic techique 44–5
ascitic fluid 280
aspartate aminotransferase 643, 646
assays
fixed change 584
fixed time 584immunoradiometric (IRMAs) 288
substrate 610–11
association constant 586, 667, 672
asymmetric peaks 441
atherosclerosis 719–22
atomic
absorption spectra 516–19
emission spectra 516–19
fluorescence spectrophotometry 519
number 554
spectroscopy 516–19
ATP as allosteric effector 599, 616,
618–19
audit (clinical laboratories)
637–9
autoanalysers 632–5
autonomous replicating sequence
elementseeARS element
autoprotolysis constant 7
autoradiography 175, 571–3
autotroph 68
bacteria (disruption of) 314
bacterial artificial chromosomes 220
bacterial cell culture 68–71
bacterial infection 712
bacterial lawn 213
bacteriophage
l211–16
vectors 211–20
band width 489
baseline
drift 442
noise 442
base-pairing (DNA) 141
basic ion-exchanger 459
batch culture 69–70
B cells 265
B-DNA 142
bead mill 315
Beer–Lambert law 486
beta particles 555
biased agonists 693
bicinchoninic acid assay 310
binding
constant 667
site 661
specific 682
bioinformatics 170–2
736