Methods in Molecular Biology • 16 Enzymes of Molecular Biology

112 Pingoud, Alves, and Geiger

Table 2

Structural Properties of Selected Restriction Enzymes

Subunit

Enzyme Amino acid res. Mol wt, kDa Reference

BcnI 211 24

BsuBI 311 43

BsuFI 395 46
BsuRI 576 66
DdeI 240 28
DpnI 254 30
DpnlI 288 34
EcoRI 276 31

EcoRII 402 45

EcoRV 245 29
FokI 583 67

HhaII 227 26
HinclI 257 30

HinfI 272 31
HpaI 254 30

MspI 262 30
MvaI 259 30

PaeR7 246 27
PstI 326 37
RsrI 276 31
Sau96I 268 31
SinI 230 27
SmaI 247 29
TaqI 234 28
TthHB 81 263 31

Butkus, Padegimiene,

Menkevicius, Butkiene,

Timinskas, and Janulaitis,

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Xu, Kapfer, Walter, and Trautner,

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Kapfer et al., 1991 (31)

Kiss et al., 1985 (32)

Sznyter et al., 1987 (33)

Lacks et al., 1986 (34)

Lacks et al., 1986 (34)

Greene et al., 1981 (35);

Newman et al., 1981 (36)

Kossykh et al., 1989 (37);

Bhagwat et al., 1990 (38)

Bougueleret et al., 1984 (39)

Kita et al., 1989 (40); Looney et

al., 1989 (41)

Schoner et al., 1983 (42)

Waite-Rees, Moran, Slatko,

Honstra-Coe, and Benner,

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Chandrasegaran et al., 1988 (43)

Waite-Rees, Moran, Slatko,

Honstra-Coe, and Benner,

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Lin et al., 1989 (44)

Xu, Kapfer, Walter, and Trautner,

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Theriault et al., 1985 (45)

Walder et al., 1984 (46)

Stephenson et al., 1989 (47)

Szilak et al., 1990 (48)

Karreman and de Waard, 1988 (49)

Heidmann et al., 1989 (50)

Slatko et al., 1987 (51)

Zebala, Mayer, and Barany,

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