Methods in Molecular Biology • 16 Enzymes of Molecular Biology

(Nancy Kaufman) #1

Nucleases--An Overview 3


Table 1
The Nomenclature of Nuclease
EC number Reaction Example
3.1. I 1 Exodeoxyribonucleases producing Exonuclease III
5'-phosphomonoesters. EC 3.1.11.
3.1.13 Exoribonucleases producing Exo-RNase H
5'-phosphomonoesters. EC 3.1.13.
3.1.14 Exoribonucleases producing Yeast RNase
other than 5'-phosphomonoesters. EC 3.1.14.
3.1.15 Exonucleases active with either Venom
ribo- or deoxyribonucleic acids exonuclease
and producing EC 3.1.15.
5'-phosphomonoesters.
3.1.16 Exonucleases active with either ribo-
deoxyribonucleic acids and
producing other than
5'-phosphomonoesters.
3.1.21 Endodeoxyribonucleases producing
5'-phosphomonoesters.

3.1.

3.1.

3.1.

3.1.

3.1.

3.1.

Endodeoxyribonucleases producing
other than 5'-phosphomonoesters.
Site-specific endodeoxyribonucleases:
specific for altered bases.
Endoribonucleases producing
5'-phosphomonoesters.
Endoribonucleases producing
other than 5'-phosphomonoesters.
Endonucleases active with either
ribo- or deoxyribonucleic acids and
producing 5'-phosphomonoesters.

Endonucleases active with either
ribo- or deoxyribonucleic acids
and producing other than
5'-phosphomonoesters.

Spleen
exonuclease
EC 3.1.16.

DNase I
EC 3.1.21.
Type II
restriction DNases
EC 3.1.21.
DNase II
EC 3.1.22.

RNase H
EC 3.1.26.
RNase T
EC 3.1.27.
Aspergillus
nuclease S1 and
Mung bean nuclease
EC 3.1.30.
Micrococcal
nuclease
EC 3.1.31.
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