Restriction Enzymes 195
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synthetic DNA duplexes as substrates for the restriction endonucleases HpalI
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cloning, purification and characterization of the EcoRV modification methy-
lase. Nucleic Acids Res. 16, 3705-3720. - Yanofsky, S. D., Love, R., McClarin, J. A. Rosenberg, J. M., Boyer, H. W., and
Greene, P. J. (1987) Clustering of null mutations in the EcoRI endonuclease.
Proteins: Struct. Funct. Genet. 2, 273-282. - Geiger, R., Riiter, T., Alves, J., Fliess, A., Wolfes, H., Pingoud, V., Urbanke,
C., Maass, G., Pingoud, A., Dtisterhrft, A., and Krrger, M. (1989) Genetic
engineering of EcoRI mutants with altered amino acid residues in the DNA
binding site: physicochemical investigations give evidence for an altered
monomer/dimer equilibrium for the Gin 144Lys 145 and Gin 144Lys 145Lys200
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Greene P. J. (1989) Determinants of EcoRI endonuclease sequence discrimi-
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A. (1990) Probing the function of individual amino acid residues in the DNA
binding site of EcoRI restriction endonuclease by analysing the toxicity of
genetically engineered mutants. Gene, 89, 19-27. - Nelson, M. and McClelland, M. (1989) Effect of site-specific methylation on
DNA. Modification methyltransferases and restriction endonucleases. Nucleic
Acids Res. 17, r389-r415. - Song, Y.-H., Rtiter, T., and Geiger, R. (1988) DNA cleavage by AatI and
StuI is sensitive to Escherichia coli d~m methylation. Nucleic Acids Res.
16, 2718. - Waalwijk, C. and Flavell R. A. (1978) MspI, an isoschizomer of HpalI which
cleaves both unmethylated and methylated HpalI sites. Nucleic Acids Res. 5,
3231-3236. - Sneider, T. W. (1980) The 5'-cytosine in CCGG is methylated in two eucary-
otic DNAs and MspI is sensitive to methylation at this site. Nucleic Acids
Res. 8, 3829-3840. - Lacks, S. A. and Greenberg, B. (1977) Complementary specificity of restric-
tion endonucleases of Diplococcus pneumoniae with respect to DNA methy-
lation. J. Mol. Biol. 114, 153-168. - Polisky, B., Greene, P., Garfin, D. E., McCarthy, B. J., Goodman, H. M., and
Boyer, H. W. (1975) Specificity of substrate recognition by the EcoRI restric-
tion endonuclease. Proc. Nat. Acad. Sci. USA 72, 3310-3314. - Hsu, M. and Berg, P. (1978) Altering the specificity of restriction endonu-
clease: effect of replacing Mg 2÷ with Mn 2÷. Biochemistry 17, 131-138.