Methods in Molecular Biology • 16 Enzymes of Molecular Biology

(Nancy Kaufman) #1
226 Maunders

The temperature of reaction must be optimized for the specific appli-
cation, conditions ranging from 37°C for 30 min to 4°C overnight.
After this time, the reaction should be terminated by boiling for 2 min.


  1. Summary
    DNA and RNA ligases are widely occurring, readily available, and
    relatively inexpensive enzymes that are an essential part of the molecular
    biologist's armory, both for the analysis and the manipulation of nucleic
    acids. The major drawback of these enzymes lies in their relative lack
    of substrate specificity as far as nucleotide sequence is involved. This
    factor necessitates careful experimental design in order to obviate the
    production of spurious or misinterpreted results.


References


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  9. Boyce, R. P. and Howard-Flanders, P. (1964) Release of ultraviolet light induced
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  10. Johnston, L. H. and Nasmyth, K. A. (1978) Saccharomyces cerevisiae cell
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  11. Panasenko, S. M., Cameron, J. R., Davis, R. W., and Lehman, I. R. (1977)
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