RNase A 269
- Incubate at 37°C for 15-30 min.
l 1. Extract protein with an equal volume phenol:chloroform solution. - Retain aqueous supernatant and add 10-20 lag of carrier tRNA.
- Precipitate the nucleic acids 2.5 vol with ethanol and 1/40 vol 4M ammo-
nium acetate. - Analyze labeled fragments by electrophoresis with a suitable polyacry-
lamide gel to detect the expected size range of fragments.
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