360 IndexCfr9I, 141, see also Restriction enzymes
CfrI, 141, see also Restriction enzymes
Chymotrypsin (EC 3.4.21.1 ), 278-281
assay, 280
ATEE, 280, 307
DFP, 279
diisopropylphosphofluoridate, see DFP
inhibitors, 281
molecular weight, 280
N-acetyl-L-tyrosine ethly ester, see
ATEE
pH optimum, 280
phenylmethane sulfonyl fluoride, see
PMSF
phenylmethyl sulfonyl fluoride, see
PMSF
PMSF, 279
specificity, 279
stability, 280
tosyl phenylalanyl chloromethyl
ketone, see TPCK
TPCK, 281
ClaI, 141, see also Restriction enzymes
Clostripain (EC 3.4.22.8), 281-283
assay, 282
BAEE, 282
benzoyl arginine ethyl ester, see BAEE
clostridiopeptidase B, 281-283
Clostridium histolyticum, 281
inhibitors, 282
molecular weight, 282
pH optimum, 282
specificity, 281
stability, 282
sulfhydryl protease, 281
Cofactors, see each enzyme
Csp45I, 141, see also Restriction
enzymes
Csp6I, 141, see also Restriction enzymes
CvnI, 141, see also Restriction enzymes
CPY, see Carboxypeptidase YDDdeI, 141, see also Restriction enzymes
Deoxyribonucleases, 7-16
DEPC, 84
DFP, 274, 279
Distamycin A, 123, 124
DNase I (EC 3.1.21.1), 8, see also
deoxyribonuclease
activators and inhibitors, 8
alkylation, 13
assay, 12
Ca 2+, Mg 2÷, 8, 10
degradative uses, 14
dI:dC, 11
DNA footprinting, 14
DNase activity, removal of, 12
DNase I protection, 14
ds DNA, 10
experimental, 11
hydrolysis, 10
K m, 10
kinetics, 9
Mn z÷, 10
nick translation, 14
pH optimum, 8
poly (dG:dC), 11
random oligonucleofide fragments, 14
reaction, 8
RNase, removal of from DNase, 13
single-stranded DNA, 10
specificity, 11
synergism, 8
transacting factors, 14
Vma x, 10
DNA ligases, 222
activators and inhibitors, 217
B. subtilis, 215
blunt termini, 220
cations, 216
cofactors, 215
cohesive termini, 219
E. coli DNA ligase, 214
enzyme assay, 218
enzyme reaction, 214
experimental procedures, 219
ligation products, 221
pH, 216
reaction conditions, 219-221
reaction protocol, 222