Methods in Molecular Biology • 16 Enzymes of Molecular Biology

Index 361

S. typhimurium, 215
storage and stability, 218
substrate, 214
sulfhydryl reagents, 217
T. thermophilus, 215
temperature, 216
uses, 219
UV damages DNA, 213
DNA methyltransferases, 201-211
5-d-Azacytidine, 210
ATP, 204
applications, 206
assay, 205
buffers, 205
cofactors, 203
construction of gene libraries, 207
CpG, see also modifying sites
Dam, 203
dA methylation, 202
dC methylation, 202
deoxycytosine, see also dc
EcoRI Mtase, 203
enzyme requirements, 203-205
experimental procedures, 205
in vitro methylation, 208
ionic strength, 204
r, 203
KC1, 204
Mg 2÷, 204
modifying sites, 201,207
NaC1, 204
pH, 205
quantitative estimation, 206
S-adenosylmethionine (SAM), 202-204
TaqI Mtase, 204
temperature, 205
template DNA, 203
transformation, 210
Type II, III, 204
DNA polymerases, 17-30
exonuclease activity 3'-5', 18
Klenow, 22, 34
enzyme (Klenow), 22
fill in reaction, 22, 23
nick translation, 22
oligolabeling, 22

strand-specific probes, 22

uses of, 22

T4 DNA polymerase, 25

cohesive termini, 24

random hexameric primers, 23

uses of, 25

T7 DNA polymerase, 26

uses of T7 DNA, 27

Taq polymerase, 27, 31

uses, 19

cDNA synthesis, 20

end labeling, 20

fill in, 18, 20

nick translation, 19

protocol for nick translation, 20, 21

repair synthesis, 20

reverse transcriptase, 20

DpnI, 141, see also Restriction enzymes

DraI, 141, see also Restriction enzymes

DralI, 141, see also Restriction enzymes

DralII, 141, see also Restriction enzymes

DrdI, 143, see also Restriction enzymes

DsaI, 142, see also Restriction enzymes

DsaV, 142, see also Restriction enzymes

E

EaeI, 142, see also Restriction enzymes

EagI, 142, see also Restriction enzymes

EarI, 142, see also Restriction enzymes

Ecl13611, 142, see also Restriction

enzymes

EcIXI, 142, see also Restriction enzymes

Ecol05I, 143, see also Restriction

enzymes

Eco l 30I, 143, see also Restriction

enzymes

Eco147I, 142, see also Restriction

enzymes

Eco24I, 142, see also Restriction enzymes

Eco31I, 142, see also Restriction enzymes

Eco47I, 142, see also Restriction enzymes

Eco47III, 142, see also Restriction

enzymes

Eco52I, 142, see also Restriction enzymes

Eco57I, 143, see also Restriction enzymes