Methods in Molecular Biology • 16 Enzymes of Molecular Biology

(Nancy Kaufman) #1
364 Index

Nlal]I, 152, see also Restriction enzymes
NlalV, 152, see also Restriction enzymes
NotI, 153, see also Restriction enzymes
NruI, 153, see also Restriction enzymes
NsiI, 153, see also Restriction enzymes
Nsp7524I, 153, see also Restriction
enzymes
Nsp7524III, 153, see also Restriction
enzymes
Nsp7524V, 153, see also Restriction
enzymes
NspI, 153, see also Restriction enzymes
NsplI, 153, see also Restriction enzymes
NsplII, 153, see also Restriction enzymes
NsplV, 153, see also Restriction enzymes
NspV, 153, see also Restriction enzymes
NspBII, 153, see also Restriction
enzymes
NspHI, 153, see also Restriction enzymes
Nucleases, 1-6
Aspergillus, 3
base specificity, 1, 2
classification, 4
cleavage, 4,5
DNases, 3
Endo-DNases, 3
exonuclease III, 3
exonucleases, 3
hairpin loop, 5
ions, 5
mung bean nuclease, 3
nomenclature, 2
nuclease S 1, 3
ribonucleases (RNases), 3
S 1 nuclease, 3
NunlI, 153, see also Restriction enzymes


O


Optimum reaction conditions, see each
enzyme


P


PaeR7I, 153, see also Restriction
enzymes
PalI, 153, see also Restriction enzymes


Pancreatic ribonuclease, 263, see also
Ribonuclease
amino acid sequence, 263
anions, 266
assay, 264, 265
double-stranded RNA, 265, 266
single-stranded RNA, 265
substrate, 265
cations, 266
DNase 1,267
disulfide bridges, 267
enzyme, 263
experimental procedures, 267-269
glycosylate ion, 264
inhibitors, 266, 267
ionic strength, 266
ions, 266
mismatch analysis, 268
molecular mass, 263
pH, 266
placental RNase inhibitor, 267
poly A RNA, 265
presecretory form, 264
proteins, 267
removal from DNase, 267
specificity, 264
stability, 267
the reaction, 264
Pancreatopeptidase E, see Elastase
Pepsin (EC 3.4.23.1), 290, 291
assay, 291
gastric proteinases, 290
inhibitors, 291
molecular mass, 291
pepsin A, 290
pH optimum, 291
specificity, 291
stability, 291
Peptidase D, 324
activation, 325
assay, 325
experimental procedures, 325
inhibition, 325
molecular mass, 324
N-terminal sequence determination,
326
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