Index 365peptide/protein hydrolysate, 326
pH optimum, 324
removal of pyroglutamic acid, 325
specificity, 324
stability, 325
PflMI, 154, see also Restriction enzymes
pH optimum, see each enzyme
PleI, 154, see also Restriction enzymes
PmaCI, 154, see also Restriction
enzymes
PmlI, 154, see also Restriction enzymes
PMSF, 279, 287
PNK, see Polynucleotide kinase
Polynucleotide kinase, 343-356
activators, 347
ATP: 5' dephosphopolynucleotide 5'
phosphatase, 343
assay, 348
blunt or recessed, 351
cations, 346
donor, 345
double-stranded DNA, 345
enzyme, 344
exchange reaction, 352
experimental procedures, 348
inhibitors, 347
kinasing DNA duplexes, 351
kinasing or duplexes single-stranded,
351
labeling oligonucleotides, 352
pH, 346
PNK, 343
protocols, 350
reaction, 344
reaction conditions, 349
RNA, 345
single-stranded DNA, 345
storage and stability, 349
substrate, 345
sulfhydryl reagents, 348
synthetic oligonucleotide, 345
temperature, 346
unit definition, 348
uses of, 348
PpuMI, 154, see also Restriction
enzymes
Porcine pancreas, 313
Prokaryotic DNA polymerase, 18, see
also DNA polymerase
Pol I, 18, see also DNA polymerase
Pol II, 18, see also DNA polymerase
Pol III, 18, see also DNA polymerase
Pol m holoenzyme, 18, see also DNA
polymerase
T4, 18, see also DNA polymerase
T7, 18, see also DNA polymerase
Prolidase, 324, 325
Proline dipeptidase, 324
Pronase, 271-276
assay, 272
DFP, 274
diisopropyl phosphofluoridate, see DFP
DNA isolation, 274
experimental procedure, 274
inhibitors, 274
Kaken, 273
molecular mass, 272
pH optimum, 272
protein hydrolysis, 274
PUK (Proteolytic Units), 273
purification, 272
serine type proteases, 271
specificity, 272
stability, 273
Streptomyces griseus, 271
Zn 2+ carboxypeptidase, 271
Zn 2÷ endopeptidases, 271
Zn 2÷ leucine aminopeptidases, 271
Proteinase K, 305-311
ATEE, 307
assay, 307
enzyme data, 306
experimental procedures, 308
nucleic acid isolation, 308
protein studies, 308
inhibitors, 308
isoelectric point, 306
molecular mass, 306
N-acetyl-L-tyrosine ethylester, see
ATEE
pH optimum, 306
specificity, 306