Methods in Molecular Biology • 16 Enzymes of Molecular Biology

366 Index

stability, 307
Tritirachium album, 305
Proteolytic enzymes, 277-303
chymotrypsin, 278-281
clostripain, 281-283
Edman degradation, 277
elastase, 283,284
endoproteinase Arg-C, 284
endoproteinase Asp-N, 286
endoproteinase Glu-C (EC 3.4.21.9),
287-289
endoproteinase Lys-C, 289
experimental procedures, 294
buffer, 294
chymotrypsin, 297
clostripain, 298
digestion conditions, 297-299
elastase, 298
endoproteinase Arg-C, 298
endoproteinase Asp-N, 298
endoproteinase Glu C, 298
endoproteinase Lys C, 298
limited digestion, 296
pepsin, 298
reaction conditions, 295
substrate, 295
termination of the digestion, 296
thermolysin, 298
total digestion, 296
trypsin, 299
pepsin (EC 3.4.23.1), 290, 291
peptide production, 277-303
proteolysis, 277
sequence determination, 277
thermolysin, 291-293
Trypsin (EC 3.4.21.4), 293, 294
PssI, 154, see also Restriction enzymes
PstI, 154, see also Restriction enzymes
Purification, see each enzyme
ATP:5' dephosphopolynucleotide 5'-
phosphatase, see Poly-
nucleotide kinase
PNK, see Polynucleotide kinase
PvuI, 155, see also Restriction enzymes
PvuII, 155, see also Restriction enzymes
Pyrococcus furiosus, 27

Pyroglutamase, 321

activation, 322

assay, 321

6-napthylamine, 321

inhibition, 322

L-pyroglutamic acid 6-

naphthylamide, 321

molecular mass, 321

oxidation, 322

pH optimum, 321

specificity, 321

stability, 322

R

Reaction conditions, see each enzyme

Restrictions enzymes, 107-200

1-cyclohexyl-3-(2-morpholinoethyl)

carbodiimide, 123

3'-exonuclease assay, 165

5,5'-dithiobis(2-nitrobenzoic acid),

123, 124

5'-exonuclease/phosphatase assay,

163, 164

actinomycin D, 123, 124

affinity chromatography, 111, 187

affinity to DNA, 115-117

AMV polymerase, 123

analysis of cleavage products, 174, 175

analytical applications, 175-183

anthramycin, 123, 124

antibiotics, 124

assay, 126--128

assays for purity, 163-166

blunt ends, 110

chain terminator, 183

chemical modification, 119, 123, 124

Cibacron dye, 111

classification, 108

cordycepin triphosphate, 183

cytostatic drugs, 124

dam methyltransferase, 121,166-173

dcm methyltransferase, 121,166-173

denaturation, 174

deoxyuridine, 120

distamycin A, 123, 124