Contents
Preface ....................................................................................................................... v
Contributors ............................................................................................................ ix
CH. 1.CH. 2.CH. 3.CH. 4.CH. 5.CH. 6.CH. 7.CH. 8.CH. 9.CH. 10.CH. 11.CH. 12.CH. 13.C,. 14.Nucleases: An Overview,
A. Fred Weir ....................................................................................... 1
Deoxyribonuclease I (EC 3.1.21.1) and II (EC 3.1.22.1),
A. Fred Weir ....................................................................................... 7
DNA Polymerases (EC 2.7.7.7),
Martin J. Maunders ......................................................................... 17
Taq Polymerase (EC 2.7.7.7): With Particular Emphasis on Its Use
in PCR Protocols,
Axel Landgraf and Heiner Wolfes .................................................. 31
Eukaryotic Nuclear RNA Polymerases (EC 2.7.7.6),
Deborah A. Cook and Robert J. Slater ........................................... 59
Reverse Transcriptase (EC 2.7.7.49): The Use of Cloned Moloney
Murine Leukemia Virus Reverse Transcriptase to Synthesize
DNA from RNA,
Gary F. Gerard and James M. D'Alessio ....................................... 73
Terminal Deoxyribonucleotidyl Transferase (EC 2.7.7.31),
Frank Grosse and Andreas Manns ................................................. 95
Restriction Enzymes,
Alfred Pingoud, Jiirgen Alves, and Robert Geiger ...................... 107
DNA Methyltransferases (EC 2.1.1.72 and EC 2.1.1.73),
David P. Hornby ............................................................................. 201
DNA and RNA Ligases (EC 6.5.1.1, EC 6.5.1.2, and EC 6.5.1.3),
Martin J. Maunders ....................................................................... 213
The BAL 31 Nucleases (EC 3.1.11),
Horace B. Gray, Jr. and Tao Lu ................................................... 231
Mung-Bean Nuclease 1 (EC 3.1.30.1),
Andrew J. Sharp and Robert J. Slater .......................................... 253
RNase A (EC 3.1.27.5),
Michael M. BurreU ........................................................................ 263
Pronase (EC 3.4.24.4),
Patricia J. Sweeney and John M. Walker .................................... 271vii