Food Biochemistry and Food Processing (2 edition)

BLBS102-c03 BLBS102-Simpson March 21, 2012 11:56 Trim: 276mm X 219mm Printer Name: Yet to Come

3 Enzymes in Food Analysis 41

Table 3.1.Some Commercial ELISA Kits for Protein or Allergen Analysis

Protein Source Target Protein ELISA Test Kit Supplier

Milk β-Lactoglobulin, caseins RIDASCREENβ-Lactoglobulin

RIDASCREEN Casein

Milk Protein ELISA Kit

Allergens ELISA Kits

FASTKIT SLIM Milk Allergens

ELISA Systemsβ-Lactoglobulin

R-Biopharm

R-Biopharm

Crystal Chem Inc

Astori Labs

Cosmo Bio USA

ELISA Systems

Nuts Peanut (Ara h1 and Ara h2) RIDASCREEN FAST Peanut

Peanut Protein ELISA Kit

Allergens ELISA Kits

FASTKIT SLIM Peanut Allergens

Peanut Residue ELISA

R-Biopharm

Morinaga Inst.

Crystal Chem Inc

Astori Labs

Cosmo Bio USA

ELISA Systems

Eggs Ovomucoid, ovalbumin,

ovotransferrin, lysozyme

RIDASCREEN FAST Egg Protein

Egg Protein ELISA Kit

Allergens ELISA Kits

FASTKIT SLIM Egg Allergens

Enhanced Egg Residue Assay

R-Biopharm

Crystal Chem Inc

Diagnostic Automation

Astori Labs

Cosmo Bio USA

ELISA Systems

Cereals Gliadins, secalin, hordein,

prolamin, gluten

RIDASCREEN FAST Gliadin

Gliadin ELISA Kit

Wheat Protein ELISA Kit

Gluten ELISA Kits

FASTKIT SLIM Wheat Allergens

ELISA Systems Gliadin

R-Biopharm

Morinaga Inst.

Crystal Chem Inc

ELISA Technologies

Astori Labs

Cosmo Bio USA

ELISA Systems

Mustard Mustard protein RIDASCREEN FAST Senf/Mustard

Allergens ELISA Kits

Veratox for Mustard Allergen

Mustard Seed Protein Assay

R-Biopharm

Astori Labs

Neogen

ELISA Systems

Wine, juice Lupine (e.g.,γ-conglutin) RIDASCREEN FAST Lupine

Allergens ELISA Kits

ELISA Systems Lupine Residue

R-Biopharm

Astori Labs

ELISA Systems

Crustaceans Tropomyosin RIDASCREEN FAST Crustacean

Allergens ELISA Kits

Crustacean Residue ELISA

R-Biopharm

Astori Labs

ELISA Systems

Soybean Soy trypsin inhibitor Soy Protein ELISA Kit

Allergens ELISA Kits

FASTKIT SLIM Soybean Allergens

US Biologicals

Diagnostic Automation

Astori Labs

Cosmo Bio USA

ELISA Systems

Potentiometric, amperometric, electrochemical, and piezoelec-

tric detection methods have since been developed and widely

used for these analyses.

Competitive ELISA is more flexible and applicable to anal-

ysis of small as well as large molecules. In the direct form

of the assay, the analyte of interest is first incubated together

with the enzyme-labeled antibody and the mixture added to the

analyte-specific capture antibody with which they react compet-

itively and in a concentration-dependent manner for detection

(Figure 3.2). The indirect form of competitive ELISA involves

co-incubation of the bound analyte of interest with an analyte-

specific antibody. Analyte detection is then achieved by intro-

ducing a second enzyme-labeled antibody into the mix, which

binds to the analyte-specific antibody. In another form of in-

direct competitive ELISA, the analyte-specific antibody is first

bound to the surface, and the analyte or protein of interest is

made to compete with an enzyme-labeled analyte or a tracer for

binding to the antibody. In all cases, the binding is concentration

dependent and can be measured by the changes in absorbance

or some other signal.