Food Biochemistry and Food Processing (2 edition)

BLBS102-c03 BLBS102-Simpson March 21, 2012 11:56 Trim: 276mm X 219mm Printer Name: Yet to Come

44 Part 1: Principles/Food Analysis

Table 3.2.Enzymatic Methods for Food Component Analysis

Food Component Enzymes Involved Commercial Kit Reference

Amino Acids

Amino acids Amino acid decarboxylase, amino

acid oxidase Wiseman 1981, Mello and

Kubota 2002,

Prodromidis and

Karayannis 2002, Cock

et al. 2009

Tryptophan Tryptophanase

Aspartic acid Aspartate aminotransferase

Glutamic acid Glutamate dehydrogenase, glutamate

oxidase, diaphorase

Megazyme International

Lysine Lysine oxidase

Carbohydrates

Sucrose Invertase, hexokinase,

glucose-6-phosphate

dehydrogenase

Megazyme International

Sigma Aldrich, Inc.

Fructose Hexokinase, phosphoglucose

isomerase, glucose-6-phosphate

dehydrogenase, fructose

dehydrogenase

Sigma Aldrich, Inc.

Megazyme

International

Glucose Glucose oxidase, peroxidase,

hexokinase, glucose-6-phosphate

dehydrogenase

Cayman Chemical Co.

Sigma Aldrich, Inc.

Megazyme

International

Wiseman 1981, Mello and

Kubota 2002,

Prodromidis and

Karayannis 2002, Cock

et al. 2009

Lactose β-Galactosidase Megazyme International

Lactulose Fructose dehydrogenase,

β-galactosidase

Raffinose α-Galactosidase, galactose

mutarotase,β-galactose

dehydrogenase

Megazyme International

Starch α-Amylase, amyloglucosidase,

glucose oxidase, peroxidase,

amyloglucosidase, hexokinase,

glucose-6-phosphate

dehydrogenase

Megazyme International

Sigma Aldrich

Company

Dietary fiber, dextran,

and hemicellulose

α-Amylase, protease,

amyloglucosidase, dextranase,

hemicellulase

Sigma Aldrich, Inc.

Pectin Pectate lyase Megazyme International

Lipids

Triglycerides Lipase, glycerol kinase, glycerol

phosphate oxidase, peroxidase,

lipoprotein lipase, glycerol kinase,

glycerol phosphate oxidase

Cayman Chemical Co.

Sigma Aldrich, Inc.

Cholesterol Cholesterol esterase, cholesterol

oxidase, peroxidase

Cayman Chemical Co.

Glycerol Glycerol kinase, glycerol phosphate

oxidase, peroxidase, glycerol

dehydrogenase,

glycerol-1-phosphate

dehydrogenase, pyruvate kinase,

lactate dehydrogenase

Megazyme International

Cayman Chemical Co.

Sigma Chemical Co.

Wiseman 1981, Mello and

Kubota 2002,

Prodromidis and

Karayannis 2002, Cock

et al. 2009

Phosphatidyl choline

(lecithin)

Phospholipase D, choline oxidase,

peroxidase

Cayman Chemical Co.

Fatty acids Lipases