Food Biochemistry and Food Processing (2 edition)

(Steven Felgate) #1

BLBS102-c42 BLBS102-Simpson March 21, 2012 14:27 Trim: 276mm X 219mm Printer Name: Yet to Come


814 Part 8: Food Safety and Food Allegens

commercial allergens detection kits appear to be adequate for
allergic consumer protection. There continues to be a need, how-
ever, for more sensitive and easily applicable analytical meth-
ods and improved understanding of the sources of cross-contact
along the food value chain.
Currently, ELISA-based rapid allergens detection kits includ-
ing LFA and dipstick are extensively used to detect suspected
major allergens in processed foods. They will likely remain the
method of choice in the food industry for a while, because of
their fast and easy usage. Other methods such as surface plas-
mon resonance on automated platforms or protein microarrays
in combination with immunochemical analytical approaches can
provide rapid, efficient and simultaneous detection of multiple
allergens in multiple samples (Moreno-Bondi et al. 2003 and
Schubert-Ullrich et al. 2009). However, application of these
novel techniques to food allergen analyses is still limited and
will hopefully grow. Similarly, use of proteomics approach to
identify and detect food allergens is expected to increase as mi-
crofluidic system-based protein separation and analysis systems
become available as handheld devices in the near future. It is
also anticipated that DNA-based food allergen detection meth-
ods will continue to be used as a valuable reinforcement tool in
the ongoing effort to manage food allergens in the food supply.

ACKNOWLEDGEMENT


The help of Dr. Sahul H. Rajamohamed in the preparation and
revision of this manuscript is gratefully acknowledged.

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