15.2 Individual Constituents 689
Table 15.18.
Sequence comparison
a
of an
α
-gliadin (clone 1235),
γ-gliadin (clone genesA), and a LMW subunit (clone LMWG-1D1)
N-terminal
α-
1
VRVPVPQLQPQNPSQQQPQEQVPLMQQQQQFPG
sequences
γ-
1
NMQVDPFGQVQWP–QQQPVLL
LMW
1
R
CCC
IPGLERPW
Recurring
α−
Gliadin
γ-Gliadin
LMW
sequences
34
QQEQ–FP–PQQPYP
11
QQQPLPP
b 1
46
HQQP–FP–SQQPYP
21
PQQPFSQ
18
QQT–FP
58
QPQP–FP–PQLPYP
28
QPQQTFPQ
23
QQPLFS
70
QTQP–FP–PQQPYP
36
PQQTFPH
29
QQQQQQL–FP
82
QPQPQYPQPQQPIS
43
QPQQQFPQ
38
QQPSFS
51
PQQPQQQFLQ
44
QQQPPFW
61
PQQPFPQ
51
QQQPPFS
68
QPQQPYPQ
58
QQQPILP
76
QPQQPFPQ
65
QQPPFS
84
TQQPQQLFPQ
71
QQQQLVLP
94
SQQPQQPYPQ
79
QQPPFS
104
QPQQPFPQ
84
QQQQPVLP
112
TQQPQQQFPQ
93
PQQSP–FP
122
SQQPQ–PFPQ
100
QQQQQH
131
PQQPQQSFPQ
106
QQLV
141
QQPS
110
QQQ–I–P
Poly-Gln
α-
96
QQQAQQQQQQQQ
sequences (II)Sequences
α-
108
TLQQILQQQLIP
CCC
RDVVLQQHNIAHASSQVL-----QQSSY
low in Pro
γ-
145
FIQPSLQQQLNP
CCC
KNLLLQQ
CCC
RPVSLVSSLW–SMIWPQSA
CCC
III
LMW
115
VVQPSILQQLNP
CCC
KVFLQQQ
CCC
SPVAMPQRLARSQMLQQSS
CCC
α-
QQLQQL
CC
QQLFQIPEQSR
CCC
QAIHNVVHAIIL
γ-
QVMRQQ
CC
QQLAQIPQQLQ
CCC
AAIHSVVHSISM
LMW
HVMQQQ
CC
QQLPQIPQQSRYEAIRAIIYSIIL
Sequences
α-
176
HHHQQQQQQPSSQVSYQQPQEQYPSGQVSFQSSQQN
high in Gln
γ-
216
QEQQQQQQQQQQQQQQQGMRILLPLYQQQQVGQGTL
IV
LMW
188
QEQQQVQGSIQSQQQQP
QQLGQCVSQPQQQSQQQLGQQPQQQQ
Sequences
α-
212
PQAQGSVQPQQLPQFQEIRNLALQTLPAM
CCC
NVYIPPY
CCC
STTIAPFG
low in Pro
γ-
253
VQGQGIIQPQQPAQLEAIRSLVLQTLPTM
CCC
NVYVPPE
CCC
SIIKAPFA
V
LMW
231
LAQGTFLQPHQIAQLEVMTSIALRILPTM
CCC
SVNVPLYRTTTSVPFG
C-terminal
α-
258
I F G T N
sequences
γ-
299
SIVTGIGGQ
LMW
277
VGTGVGAY
a
The segments of the recurring and low-proline sequences are arranged according to the best possible homology (–: space to maximize homology). Cystei
ne residues
(CCC
) are in bold type.
b
Roman numerals: division into sequence segments (cf. Fig. 15.10)