Food Chemistry

(Sean Pound) #1
15.2 Individual Constituents 689

Table 15.18.

Sequence comparison

a


of an

α
-gliadin (clone 1235),

γ-gliadin (clone genesA), and a LMW subunit (clone LMWG-1D1)

N-terminal

α-

1

VRVPVPQLQPQNPSQQQPQEQVPLMQQQQQFPG

sequences

γ-

1

NMQVDPFGQVQWP–QQQPVLL

LMW

1

R
CCC

IPGLERPW

Recurring

α−

Gliadin

γ-Gliadin

LMW

sequences

34

QQEQ–FP–PQQPYP

11

QQQPLPP

b 1

46

HQQP–FP–SQQPYP

21

PQQPFSQ

18

QQT–FP

58

QPQP–FP–PQLPYP

28

QPQQTFPQ

23

QQPLFS

70

QTQP–FP–PQQPYP

36

PQQTFPH

29

QQQQQQL–FP

82

QPQPQYPQPQQPIS

43

QPQQQFPQ

38

QQPSFS

51

PQQPQQQFLQ

44

QQQPPFW

61

PQQPFPQ

51

QQQPPFS

68

QPQQPYPQ

58

QQQPILP

76

QPQQPFPQ

65

QQPPFS

84

TQQPQQLFPQ

71

QQQQLVLP

94

SQQPQQPYPQ

79

QQPPFS

104

QPQQPFPQ

84

QQQQPVLP

112

TQQPQQQFPQ

93

PQQSP–FP

122

SQQPQ–PFPQ

100

QQQQQH

131

PQQPQQSFPQ

106

QQLV

141

QQPS

110

QQQ–I–P

Poly-Gln

α-

96

QQQAQQQQQQQQ

sequences (II)Sequences

α-

108

TLQQILQQQLIP

CCC

RDVVLQQHNIAHASSQVL-----QQSSY

low in Pro

γ-

145

FIQPSLQQQLNP

CCC

KNLLLQQ

CCC

RPVSLVSSLW–SMIWPQSA

CCC

III

LMW

115

VVQPSILQQLNP

CCC

KVFLQQQ

CCC

SPVAMPQRLARSQMLQQSS

CCC

α-

QQLQQL

CC

QQLFQIPEQSR

CCC

QAIHNVVHAIIL

γ-

QVMRQQ

CC

QQLAQIPQQLQ

CCC

AAIHSVVHSISM

LMW

HVMQQQ

CC

QQLPQIPQQSRYEAIRAIIYSIIL

Sequences

α-

176

HHHQQQQQQPSSQVSYQQPQEQYPSGQVSFQSSQQN

high in Gln

γ-

216

QEQQQQQQQQQQQQQQQGMRILLPLYQQQQVGQGTL

IV

LMW

188

QEQQQVQGSIQSQQQQP

QQLGQCVSQPQQQSQQQLGQQPQQQQ

Sequences

α-

212

PQAQGSVQPQQLPQFQEIRNLALQTLPAM

CCC

NVYIPPY

CCC

STTIAPFG

low in Pro

γ-

253

VQGQGIIQPQQPAQLEAIRSLVLQTLPTM

CCC

NVYVPPE

CCC

SIIKAPFA

V

LMW

231

LAQGTFLQPHQIAQLEVMTSIALRILPTM

CCC

SVNVPLYRTTTSVPFG

C-terminal

α-

258

I F G T N

sequences

γ-

299

SIVTGIGGQ

LMW

277

VGTGVGAY

a
The segments of the recurring and low-proline sequences are arranged according to the best possible homology (–: space to maximize homology). Cystei

ne residues

(CCC


) are in bold type.
b

Roman numerals: division into sequence segments (cf. Fig. 15.10)
Free download pdf