SCIENCEscience.org 29 OCTOBER 2021¥VOL 374 ISSUE 6567 631
Fig. 3NIN is necessary and sufficient
for early and late nodulation-associated
gene expression.(A) Genes up-regulated
byNINoverexpression (NINox) that
are also induced after treatment with
S. meliloti, shown in wild type andnin-1
mutants 12 to 168 hours and 24 hours,
respectively, afterS. melilotiinoculation
and indnf1-2andnin-16nodules (log2 fold
changes for the mutants are relative to
the wild type with the equivalent treatment).
(BandC) Heatmaps of selected genes
from (A). Lb, leghemoglobin; Trx, thioredoxin.
(D) Transactivation assays. Fluc, firefly
luciferase; Rluc,Renillaluciferase. Data are
mean ± SEM (n= 6 biological replicates).
Letters a, b, and c denote statistically
significant groupings analyzed by one-way
analysis of variance with TukeyÕs test,
P< 0.05.RESEARCH | REPORTS