Science - USA (2021-11-12)

846 12 NOVEMBER 2021¥VOL 374 ISSUE 6569 science.orgSCIENCE

S. taczanowskii

S. dalli

S. schlegelii

S. wilsoni

S. rosaceus

S. carnatus

S. inermis

S. semicinctus

S. steindachneri

S. serriceps

S. atrovirens

S. hopkinsi

S. exsul

S. minor

S. rastrelliger

S. melanops

S. zacentrus

S. flavidus

S. paucispinis

S. elongatus

S. rosenblatti

S. miniatus

S. mentella

S. caurinus

S. ciliatus

S. fasciatus

S. proriger

S. variabilis

S. entomelas

S. nebulosus

S. variegatus

S. ensifer

S. jordani

S. umbrosus

S. mystinus

S. chlorostictus

S. saxicola

S. auriculatus

S. goodei

S. constellatus

S. diploproa

S. helvomaculatus

S. aleutianus

S. babcocki

S. alutus

S. nigrocinctus

S. melanostomus

S. pinniger

S. aurora

S. maliger

S. reedi

S. ruberrimus

S. polyspinis

S. crameri

10

3

10

4

10

5

10

6

10

3

10

4

10

5

10

6

50

100

150

200

250

50

100

150

200

250

generations

N

(x10e

4 )

0.001

0.002

0.003

0.004

0.005

Heterozygosity per bp

A B

P 0.0046

50

100

150

11−3030−5252−7

9

79−200

lifespan quartile (yrs)

N

(x10e

4 )

P 6.6e−05

50

100

150

14−3030−4242−5151−64

size quartile (cm)

N

(x10e

4 )

C

D

Sebastolobus alascanus

Sebastolobus altivelis

Helicolenus avius

Hozukius emblemarius

Sebastes rosaceus

Adelosebastes latens

Sebastes umbrosus

Sebastes zacent

Sebastes mystinus

Sebastiscus tertius

Sebastes reedi

Sebastes levis

Sebastiscus albofasciatus

rus

Fig. 4. Life-history transitions are associated with patterns of diversity.(A) Nucleotide diversity across 88 different species. (B) MSMC-based estimates
of effective population sizes grouped by life span quartile over the last ~10^6 generations and averagedNegrouped by (C) life-span quartile or (D) grouped
by size quartile. Box and whisker plots indicate the median and interquartile range (IQR) with tails at the minimum and maximum values within 1.5 × IQR.
Pvalues from PGLS linear model.

Fig. 5. Shifts in life-history
traits reshape the mutational
spectrum of segregating
genetic variation.(A) Survival
curves for 34 rockfish species.
Humans, shown for comparison,
exhibit maximum life spans similar
to those of many rockfish species
but different survival curves
(type I versus type III survival).
(B) Rockfish fecundity (births per
season) plotted as a function of
age. (C) Reproductive value
plotted as a function of age.
(D) Generation times estimated
from survival and fecundity
plotted as a function of life span.
(E) Association of segregating
single-nucleotide variant mutation
types with life span across all
trinucleotide contexts. Asterisks
indicate age-associated mutational
profiles significant after multiple
testing correction (q< 0.05).
(F) The proportion of segregating
CpG→TpG mutations as a function
of maximum life span. LM,
linear regression model.

enrichment

-0.01 0.00 0.01

S. aleutianus

S. aurora

S. caurinus

S. crameri

S. flavidus

S. maliger

S. melanops

S. pinniger

S. ruberrimus

S. rufus

0

500

1000

1500

0 50 100 150 200

age

Fecundity (1000s)

Fecundity

0.00

0.25

0.50

0.75

1.00

0 50 100 150 200

age

p(survival)

S. aleutianusS. alutus

S. atrovirensS. auriculatus

S. auroraS. carnatus

S. caurinusS. chlorostictus

S. constellatusS. crameri

S. dalliS. diploproa

S. elongatusS. ensifer

S. entomelasS. flavidus

S. goodei

S. helvomaculatusS. hopkinsi

S. jordaniS. maliger

S. melanopsS. melanostomus

S. mystinusS. nebulosus

S. ovalisS. pinniger

S. rastrelligerS. rosaceus

S. rosenblattiS. ruberrimus

S. rufusS. serranoides

S. simulator

Survival

Human

0

10

20

0 50 100 150 200

age

Reproductive Value

Reproductive Value

10

20

30

40

50

50 100 150 200

Maximum lifespan

Generation time

(years)

Generation time

E 5’-

A

C

G

T

A

C

G

T

A

C

G

T

A

C

G

T

A

C

G

T

A

C

G

T

3’-AGCT

A

C

F

B

D

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