Science - USA (2021-12-10)

Shiet al.,Science 374 , eabj6641 (2021) 10 December 2021 2 of 12

3

CGE

GPM6A

Progenitors Postmitotic cells

VIM

TOP2ANUSAP1

PTTG1

NTRK2

KIAA0101

HES1

FABP7

TTYH1

SPARC

SFRP1

MEF2C

ERBB4

TAC1

RBP1

PLS3

SYT1CXCR4

DCX

SST

PDE4DIP

PCSK1N

NKX2-1

LHX6

NXPH1

PLS3

SOX6

SFTA3

MEIS2

FOXP1

SIX3

ZNF503

EBF1

ID4

SERTAD4

ISL1

TLE4

SCGN

CALB2

NR2F2

PCDH9

NFIB

NR2F1

PDZRN3

KLHL35

AP1S2

ANKS1B

MGE LGE CGE

-2 02 -2 02

GW12 NTRK2/DAPI^3

VZ

SVZ

LGE

GW12 NTRK2/DAPI

MGE

1

2

VZ

12

ic

Str

SVZ

VZ

SVZ Str

GW9

GW12

GW16

GW18

GW13

MGE CGE

GE progenitors LGE

Thalamic

neurons

OPCs

Endothelial cells

0

4

ASCL1

0

4

MKI67

0

3.5

HES1

0

4

NKX2-1

0

3

LHX6

0

3.5

SOX6

0

3.5

NR2F2

0

4

NR2F1

0

4

FOXP1

0

4

MEIS2

0

4

TCF7L2

0

3

NEUROD2

Excitatory

neurons

Progenitors

Pallial

cells

Microglia

AB

C D

E

GW18-02 (CGE)

GW18-02 (MGE)

GW18-02 (LGE)

ARHGAP11B

Fig. 1. Major sources of transcriptional heterogeneity among cells from
the human ganglionic eminences.(A) Clustering of individual cells from
different gestational weeks visualized by UMAP. (B) Annotation of cell
clusters on the basis of gene expression. The schema on the right illustrates
the distribution of cells obtained from individual dissections of the MGE, LGE,
and CGE in relation to the unsupervised clustering of all cells. (C) Gene
expression visualized by UMAP. Each dot represents an individual cell colored

according to the expression level (red, high; gray, low). (D) Heatmaps

illustrating DEGs enriched in progenitors and postmitotic cells in the

ganglionic eminences (left) and among postmitotic cells in the MGE, LGE, and

CGE (right). (E) Expression of NTRK2 in progenitor cells in the ganglionic

eminences at GW12. The areas in white boxes are shown at high magnification.

Scale bars, 200mm (LGE and MGE), 100mm (CGE), 10mm (boxes 1 to 3).

Str, striatum; ic, internal capsule; DAPI, 4′,6-diamidino-2-phenylindole.

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