Dairy Chemistry And Biochemistry

156 DAIRY CHEMISTRY AND BIOCHEMISTRY

N

X

Y

L

&-

20 40 00 80 LOO I20 I40 160 180 2w 220

Fraction number

Figure 4.5 (a) Chromatogram of sodium caseinate on an open column of DEAE cellulose
anion exchanger. Buffer: S M urea in imidazole-HCI buffer, pH 7.0; gradient: 0-0.5 M NaC1.
(b) Urea polyacrylamide gel electrophoretograms of the fractions from (a). (c) Chromatogram
of sodium caseinate on a Pharmacia Mono Q HR5/S anion exchange column. Buffer: 6 M urea
in 5 mM bis-tris-propane/7 mM HCI, pH 7; gradient: 0-0.5 M NaC1. (d) Chromatogram of
sodium caseinate on a Pharmacia Mono S HRS/S cation exchange column. Buffer: 8 M urea
in 20 mM acetate buffer, pH 5; gradient: 0-1.0 M NaC1.