cow
SHE
MOW
RAT
RAE
MANcow
SHE
MOW
RAT
RAE
MANcow
SHE
MOW
RAT
RAE
MAN10 .1 20 30 40 50 60 70 80 90 100
MKVLILACLVALALARELEELNVPGEIVESLSSSEESITRIN-KKIEKFQSEEQQQTEDELQDKIHPFAQTQSLVYPFPG--PIP-NSLPQNIPPLTQTP
MKVLILACLVALALAREQEELNWGETVESLSSSEESLSSSEESITHIN-KKIEKFQSEEQQQTEDELQDKIHPFAQAQSL~PFTG--PIP-NSLPQNILPLTQTP
MKVFILACLVALALARE-TTFTVSSET-DSI-SSEESVEHINEQKLQKVNLMGQLQAEDVLQAKVHSSIQSQPQAFPYAQAQTISCNPVPQNIQPIAQPP
MKVFILACLVALALAREKDAFTVSSET-GSI~SSEESVEHINE~KLQKVKLMGQVQSEDVLQNKFHSGIQSEP~IPYAQ--TISCSPIPQNIQPIAQPP
MKVLILACLVALALAREKEQLSVPTEAVGSVSSSEE~ITHINKQKLETIKHVEQLLREEKLQDKILPFIQS---LFPFAE--RIPYPTLPQNILNLAQLD
MKVLILACLVALALARE~~~~~~~~~TIESLSSSEESITEYK~QKEKVKHEDQQQGEDQQQGEDEHQDKIYPSFQPQPLIYP~E--PIPYGFLPQNILPLAQPA
***+************* *+ **** ++ ++ *+++++ + *+ +* *+ + * *+ +*+ **** +++*
110 120 130 140 150 160 170 180 190 200
VW--PPFLQPEVMGVSKVKEAMAPKHKEMPFPKYP-VEPFTESQSLTLTDVENLHLPLPLLQSWQPHQPLPPTVM-FPPQSVLSLSQSKVLPVPQKA
VW--PPFLQPEIMGVPKVKETMVPKHKEMPFPKYP-VEPFPKYP-VEPFTESQSLTLTDVEKLHLPLPLVQS~KQPPQPLPPTVM-FPPQSVLSLSQPKVLPVPQKA
WPSLGPVISPELESFLKAKATILPKHKQMPLLNSETVLRLINSQIPSLASLANLHLPQSLVQ-LLAQWQAFPQTHL-VSSQTQLSLPQSKVLYFLQQV
WPTDGPIISPELESFLKLP~KQMPFLNSETVLSQIPSLD-LANLHLPQSPAQ-LQAQIVQAFPQTPAWSSQPQLSHPQSKSQYLVQQL
MLL---PLLQPEIMEDPKAKETIIPKHKLMPFLKSPK?lrPFVDSQILNLREMKNQHLLLPQLLPFQVFQPFPQTPI-PYPQALLSLPQSKFMPIVPQV
WL---PVPQPEIMEVPKAKDTVYTKGRVMPVLKSP-TIPFFDPQIPKVTDLENLQLPLPLLQPLMQQVPQPIPQTLA-LP?QPLWSVPQPKVLPIPQQV
++ *+ +**++ *+* ++ +*++ **+++++ + ++ +*++ + + +++*+++ + *+ *++*+* +* +* +*+* ++ ++
210 220 230 240
VPYPQRDMPIQAFLLYQEPVLGPVRGPFPIIV
VP--QRDMPIQAFLLYQEPVLGPVRGPFPILV
APFLPQDMSVQDLLQYLE-LLNPTVQ-FPATPQHSVSV
APLFQQGMPVQDLLQYLDLLLNPTLQ-FLATQQLHSTSV
VPYPQRDMPIQALQLFQELLF-PTHQGYPWQPIAPVNV
VPYPQRAVPVQALLLNQELLLN?THQIYPVTQPL?.P”IPISV
+* ++ +++*++++ ++ ++ *+ + ++Figure 4.16 Homology of /3-casein from a selection of species; * indicates residues identical at the same position for all species; + indicates similar residues,
- an inserted space. 1. indicates the N terminus of the mature protein (from Holt and Sawyer, 1993).