49Arrayit® H25K
Arrayit® H25K is the only genome microarray based on the completely sequenced
human genome. It contains a fully annotated set of 25,509 human gene sequences
and 795 controls. H25K is a multi-purpose long oligonucleotide microarray that
allows karyotyping, gene expression profi ling, chromatin structure analysis, and
protein-DNA interaction studies on a genomic scale. Its glass substrate slide format
is fully compatible with every major microarray scanner brand including the Arrayit
InnoScan and SpotLight Scanner series.
AmpliChip CYP450
AmpliChip CYP450 (Roche Molecular Diagnostics) is combined with the GeneChip
3000Dx microarray system of Affymetrix. It was cleared by the regulatory authori-
ties for marketing in the US and the EU as an IVD test in 2004. The microarray chip
( also referred to as “probe microarray”) contains millions of tiny DNA molecules
and the test is performed using DNA that is extracted from a patient’s blood. DNA
sequence is determined based on the sequence of the probe molecule to which the
DNA is most similar. AmpliChip CYP450 contains >15,000 different oligonucle-
otide probes to analyze both the sense and the antisense strands of an amplifi ed
target DNA sample (Jain 2005 ).
By multiplexing long PCR reactions that amplify the promoter and coding region
of CYP2D6, exons 4 and 5 of CYP2C19, as well as a 3.5-kb CYP2D6 gene deletion
or CYP2D6 gene duplication-specifi c reactions, virtually all known polymorphisms
and alleles of CYP2D6, and the two most frequent for CYP2C19, can be detected
simultaneously. AmpliChip CYP450 comprehensively covers gene variations that
play a role in the metabolism of ~25 % of all prescription drugs. The product char-
acteristics are as follows:
- The chip has high built-in sensitivity for analyzing 29 polymorphisms and muta-
tions for the 2D6 gene and 2 polymorphisms for the 2C19 gene, thereby
increasing the probability of more accurately determining the genotype and phe-
notype. Accurately genotypes >99 % of the world’s population. - Analysis can normally be completed within a single 8 h shift.
- The software automatically determines genotype and predicted phenotype.
- Results are obtained and interpreted by computer analysis performed on a scan
showing the pattern of hybridization of the patient sample to a series of probes
(arrayed on the glass substrate) that are specifi cally complementary to either
wild-type or mutant sequences. - AmpliChip CYP450 test is intended to be an aid for physicians in individualizing
treatment doses for patients on therapeutics metabolized through these genes.
DNA samples that represent nine different allelic variants including 2D6 gene
deletions and duplications can be tested with the AmpliChip CYP450 microarray
Biochips and Microarrays