Analytical Chemistry

(Chris Devlin) #1

Table 4.23 Buffers commonly used in capillary electrophoresis


Name pKa


phosphate 2.12 (pKa1)


citrate 3.06 (pKa1)


formate 3.75


succinate 4.19 (pKa1)


citrate 4.74 (pKa2)


acetate 4.75


citrate 5.40 (pK
a3)


succinate 5.57 (pK
a2)


MES 6.15


ADA 6.60


BIS-TRIS propane 6.80


PIPES 6.80


ACES 6.90


MOPSO 6.90


imidazole 7.00


MOPS 7.20


phosphate 7.21 (pKa2)


TES 7.50


HEPES 7.55


HEPPS 8.00


TRICINE 8.15


glycine amide, 8.20


hydrochloride glycylglycine 8.25


TRIS 8.30


BICINE 8.35


morpholine 8.49


borate 9.24


CHES 9.50


CHAPSO 9.60

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