6B Peptides 193
Ta b l e 6 B. 1
Procedure and material used for sample preparation and techniques for separation and detection of wine peptides
Sample preparation
Technique used
Reference
Procedure
Material
Peptides separation
Peptides detection
Proteins precipitation
TCA 7%
∗
Yokotsuka et al. (1975)
↓↓
Low pressure cationic exchange
Dowex 50-X2
chromatography
Low pressure cationic exchange
Amberlite IR-H 120
Usseglio-Tomasset and
Bosia (1990)
chromatography
Ultrafiltration
Cut-off membrane 10000
RP-HPLC
Absorbance at 214 nm
and fluorescence ofthe OPA derivatives
Acedo et al. (1994)
Da
Ultrasphere ODS column
↓↓
Low pressure exclusion chromatography
Sephadex LH-20
Proteins precipitation
Ethanol 95%
RP-HPLC
Absorbance at 214 nm
Moreno-Arribas
et al. 1996, (1998a,b);Mart ́
ınez-Rodr ́
ıguez
et al. (2002)
Nova-Pak C18 column
↓↓
Low pressure exclusion chromatography
Sephadex G-10
Proteins precipitation
Ethanol 95%
RP-HPLC
Scanning from 190 to
280 nm and fluores-cence of the OPAderivatives
Bartolom ́
e et al. (1997)
Nova-Pak C18 column
↓↓
Low pressure exclusion chromatography
Sephadex G-10
Low pressure RP-chromatography
Cosmosil 140 C
18
-OPN
RP-HPLC
Absorbance at 220 nm
Takayanagi and
Yokotsuka (1999)
μ
Bondasphere C18 column
↓↓GPC
Toyopearl HW-40