Wine Chemistry and Biochemistry

(Steven Felgate) #1

6B Peptides 193


Ta b l e 6 B. 1

Procedure and material used for sample preparation and techniques for separation and detection of wine peptides
Sample preparation

Technique used

Reference

Procedure

Material

Peptides separation

Peptides detection

Proteins precipitation

TCA 7%


Yokotsuka et al. (1975)

↓↓

Low pressure cationic exchange

Dowex 50-X2

chromatography

Low pressure cationic exchange

Amberlite IR-H 120

Usseglio-Tomasset and

Bosia (1990)

chromatography

Ultrafiltration

Cut-off membrane 10000

RP-HPLC

Absorbance at 214 nm

and fluorescence ofthe OPA derivatives

Acedo et al. (1994)

Da

Ultrasphere ODS column

↓↓

Low pressure exclusion chromatography

Sephadex LH-20

Proteins precipitation

Ethanol 95%

RP-HPLC

Absorbance at 214 nm

Moreno-Arribas

et al. 1996, (1998a,b);Mart ́

ınez-Rodr ́

ıguez

et al. (2002)

Nova-Pak C18 column

↓↓

Low pressure exclusion chromatography

Sephadex G-10

Proteins precipitation

Ethanol 95%

RP-HPLC

Scanning from 190 to

280 nm and fluores-cence of the OPAderivatives

Bartolom ́

e et al. (1997)

Nova-Pak C18 column

↓↓

Low pressure exclusion chromatography

Sephadex G-10

Low pressure RP-chromatography

Cosmosil 140 C

18

-OPN

RP-HPLC

Absorbance at 220 nm

Takayanagi and

Yokotsuka (1999)

μ

Bondasphere C18 column

↓↓GPC

Toyopearl HW-40
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