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ACKNOWLEDGMENTS
We thank M. J. Danton for critically reviewing this manuscript. We
also thank the NCI microbiome sequencing core for assistance
with 16SrRNA sequencing and data deposition.Funding:This work
was funded by the NIH NIDCR Intramural Research Program (T.H.B.
and N.M.M.); NIDCR 1K99DE030124-01A1 (L.M.S.); NIDCR Veterinary
Resources Core (ZIC DE000740-05); NIDCR/NIDCD Genomics and
Computational Biology Core (ZIC DC000086); NIDCR Combined
Technical Research Core (ZIC DE000729-09); and NIDCR
U01DE025046 (C.S.A. and K.D.). This work was also supported in
part by NIH R01DK112778 and NIH R01CA211098 (M.J.F.), NIH
R01HL013423-43A1 (F.J.C.), and FONDECYT grant 11180505 (L.A.).
Author contributions:Conceptualization: L.M.S., T.H.B., and
N.M.M.; Formal analysis: L.M.S., D.M., K.M., C.S.A., and K.D.; Funding
acquisition: T.H.B. and N.M.M.; Investigation: L.M.S., A.D.D., T.G.-W.,
C.L.T., L.A., and C.E.Z.; Methodology: L.M.S., A.D.D., T.G.-W., N.D.,
C.L.T., L.A., E.M.C., A.G.L., C.E.Z., M.S., M.S.L., and M.J.F.; Project
administration: L.M.S., T.H.B., and N.M.M.; Resources: L.J.J., J.L.,
P.J., V.K., F.J.C., C.J.K., and M.J.F.; Software: A.D.D.; Visualization:
L.M.S.; Writing–original draft: L.M.S., A.D.D., K.D., T.H.B., and N.M.M.;
Writing–review and editing: all authors.Competing interests:
C.J.K. is a director and shareholder of NanoVation Therapeutics Inc.,
which is commercializing RNA-based therapies. C.J.K., L.J.J., and J.L.
are inventors on pending intellectual property related to RNA-based
therapies. The other authors declare that they have no competing
interests.Data and materials availability:RNA-seq data have been
deposited to the GEO database (record GSE178616) and can be
accessed using the token: ylkhyomevlszjuv. Sequencing data have
been deposited to the Sequence Read Archive (SRA) with the
BioProjectID PRJNA753711 (https://dataview.ncbi.nlm.nih.gov/object/
PRJNA753711?reviewer=a5barpkgib249d5v2res6mtgef). All other data
are available in the main text or supplementary materials.SUPPLEMENTARY MATERIALS
science.org/doi/10.1126/science.abl5450
Figs. S1 to S9
Tables S1 and S2
MDAR Reproducibility Checklist21 July 2021; accepted 29 October 2021
10.1126/science.abl5450Silvaet al.,Science 374 , eabl5450 (2021) 24 December 2021 11 of 11
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