Science - USA (2022-01-14)

SCIENCEscience.org 14 JANUARY 2022¥VOL 375 ISSUE 6577 187

1

10

100

1000

SFC per 10

6 PBMC

VVVaccination

SARS-CoV-2 infection

(Wuhan Hu-1)

• 
  
  
  
  • 
    
    
    
    • 
      
      
      
      • 
        
      
      
      
      
    
    
    
    
  
  
  
  

p = 0.0003 p = 0.0294

+ ++ + ++

A

B

F

J

Magnitude of gene expression

Min Average Max

DE

C

GH

1

10

100

1,000

SFC per 10

6 PBMC

n = 23

Vaccination + ++

1

10

100

1,000

SFC per 10

6 PBMC

n = 24

p = 0.0119

+ ++

1

10

100

1,000

SFC per 10

6 PBMC

n = 23

p = 0.0008

Vaccination + ++

1

10

100

1,000

SFC per 10

6 PBMC

n = 24

p = 0.0004

+ ++

I

0

100

200

300

400

400

600

800

1000

SFC per 10

6 PBMC

Wuh

Poo

l

P. 1WuhP. 1WuhP. 1WuhP. 1WuhP. 1WuhP. 1WuhP. 1WuhP. 1WuhP. 1WuhP. 1WuhP. 1WuhP. 1WuhP. 1WuhP. 1WuhP. 1WuhP. 1

L18F

/T2

0N

P80R P26SD138YR190SK417TE484KN501YD614GH655YT1027I S1188 E92K

L

K1795QE5665

D

p = 0.0256 p = 0.0006 p = 0.0064

N Spike ORF1a ORF8

n = 7

Wuhan Hu-1

B.1.1.7

1

10

100

1000

SFC per 10

6 PBMC

Wuhan Hu-1

B.1.1.7

1

10

100

1000

SFC per 10

6 PBMC

p = 0.0153

Wuhan Hu-1

B.1.351

1

10

100

1000

SFC per 10

6 PBMC

p = 0.0501

Wuhan Hu-1

B.1.351

1

10

100

1000

SFC per 10

6 PBMC

Wuhan Hu-1

P1

1

10

100

1000

SFC per 10

6 PBMC

Wuhan Hu-1

P1

1

10

100

1000

SFC per 10

6 PBMC

Wuhan Hu-1

Variant

1

10

100

1000

SFC per 10

6 PBMC

Wu

han H

u-1

Variant

1

10

100

1000

SFC per 10

6 PBMC

p = 0.0156

N501Y

Wuhan

Hu-1Variant

1

10

100

1000

SFC per 10

6 PBMC

Wuhan Hu-

1

Variant

1

10

100

1000

SFC per 10

6 PBMC

D1118H

Wu

han Hu

-^1
B.1.617.2

1

10

100

1000

SFC per 10

6 PBMC

Wuhan

Hu-

1

B.1.

6 17.2

1

10

100

1000

SFC per 10

6 PBMC

p = 0.0283

n= 23 n= 23 n= 23 n = 19 n =^23 n =^18

n = 24 n = 24 n = 24 n = 19 n = 20 n = 1 3

0

1

2

3

4

5

Relative expression of IFNg

No peptide

N501YN501

Y

p = 0.0020

p = 0.0020

0

1

2

3

Relative expression of Foxp3

N^501

Y

N50

1 Y

p = 0.0020

p = 0.0078

p = 0.0020

No peptid

e

0.0

0.5

1.0

1.5

2.0

Relative expression of IRF4

No p

eptideN^501

Y

N501

Y

p = 0.0020

p = 0.0371

p = 0.0020

n = 10 n = 10 n = 10

0

1

2

3

4

% Foxp3+ CD4 T cells

No peptide

N501YN501

Y

0.0020

0.0039

0.0020

n = 10

0

100

200

300

400

400

600

800

1000

SFC per 10

6 PBMC

Wuh

Poo

l

B.1.617.2

Spike

Wuh

B.1.617.2

Wuh

B.1.617.2

Wuh

B.1.617.2 B.1.617.2 B.1.617.2 B.1.617.2

Wuh Wuh Wuh

T^19

R

p = 0.0022 p = 0.0325

EF1

56 del T478

K

D61

4G

P681R D95

0N

n = 6 p = 0.0022

0

100

200

300

400

400

600

800

1000

SFC per 10

6 PBMC

Wuh

Poo

l

B.1.617.2

Spike

Wuh

B.1.617.2

Wuh

B.1.617.2

Wuh

B.1.617.2 B.1.617.2 B.1.617.2 B.1.617.2

Wuh Wuh Wuh

T19R

EF1

56 del T478K D^614

G

P681R D950

N

p = 0.0022 p = 0.0022 n = 6 p = 0.0022 p = 0.0065

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